Show simple item record

Insulin-induced morphological changes in the exocrine pancreas of rat

dc.contributor.advisorKorać, Aleksandra
dc.contributor.otherStančić, Ana
dc.contributor.otherKorać, Bato
dc.creatorPajević, Mila
dc.date.accessioned2021-03-17T10:27:16Z
dc.date.available2021-03-17T10:27:16Z
dc.date.issued2018-07-07
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=7974
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:23339/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=33355017
dc.identifier.urihttps://nardus.mpn.gov.rs/handle/123456789/18066
dc.description.abstractEgzokrini i endokrini pankreas ostvaruju složenu anatomsku i funkcijsku interakciju. Naime, egzokrini deo pankreasa je pod uticajem hormona endokrinog, ne samo sistemski kao ostale ćelije organizma, već i direktno, preko veze portnog sistema acinusa i Langerhansovog ostrvca. Pokazano je da i endogeni i egzogeni insulin izazivaju povećanu sintezu pankreasnih enzima u acinusima, a time i njihov rast. Veza strukturnog (ćelijskog/tkivnog) i funkcijskog odgovora egzokrinog pankreasa na periportalni nivo insulina ukazuje na visok i kompleksan stepen insulo-acinusne interakcije u fiziološkim, a pogotovo u patološkim stanjima. Cilj ove doktorske disertacije je da istraži histološke promene egzokrinog pankreasa i ultrastrukturne promene acinusnih ćelija tokom remodeliranja izazvanog insulinom. U eksperimentu su korišćeni Wistar pacovi, starosti dva meseca. Pacovi su podeljeni u šest grupa, četiri grupe su primale i.p. nisku (0.4 IU) ili visoku (4 IU) dozu insulina, jedan (akutno) ili tri dana (hronično). Preostale dve grupe na isti način primale su fiziološki rastvor i predstavljale su odgovarajuće kontrole. Tri sata nakon poslednjeg tretmana, sve životinje su žrtvovane dekapitacijom. Odmah nakon žrtvovanja, pankreas je izolovan, slikan, izmeren i odvojen za svetlosnui elektronsku mikroskopiju. Efekti insulina na histološke promene egzokrinog pankreasa analizirane su svetlosnom mikroskopijom (hematoksilin/eozin bojenje, AZAN), fraktalnom analizom, imunohistohemijom (amilaza, pHH3 – fosforilisana forma histona H3 (eng. PhosphoHistone H3), Ki-67 i PCNA – nukleusni antigen proliferišućih ćelija (eng. Proliferating Cell Nuclear Antigen)) i elektronskom mikroskopijom (volumenska gustina citoplazme, dijametar i volumenska gustina zimogenih granula, broj i volumenska gustina nukleusa)...sr
dc.description.abstractExocrine and endocrine pancreas achieves complex anatomical and functional interaction. Namely, the exocrine part of the pancreas is influenced by the endocrine hormone, not only systemically as other cells in organizm, but also directly, via the islet acinar portal vascular system. It has been shown that both endogenous and exogenous insulin cause an increased synthesis of pancreatic enzymes in the acinus and hence their growth. The relationship between the structural (cellular/tissue) and functional response of exocrine pancreas to the periportal level of insulin indicates a high and complex level of insulin-acinus interaction in the physiological and especially in the pathological state. The goal of this doctoral dissertation was to investigate the histological changes of exocrine pancreas and ultrastructural changes of acinar cells during insulininduced remodeling. Two-month-old male Wistar rats were used. Rats were divided into six groups, four groups were treated i.p. with low (0.4 IU) or high (4 IU) dose of insulin, one (acutely) or three (chronically) days. The other two groups, in the same way, were received 0.9% saline solution and served as a corresponding control. Three hours after the last injection, the animals were sacrificed by decapitation. Immediately after sacrificing, the pancreas was isolated, pictured, weighted and prepared for light and electron microscopy. The effect of insulin on the histological changes of exocrine pancreas was analyzed by light microscopy (hematoxylin/eosin staining, AZAN), fractal analysis, immunohistochemistry (amylase, pHH3, Ki67 and PCNA) and electron microscopy (volume density of cytoplasm, diameter and volume density of zymogene granules, as well as number and volume density of nuclei). Discrete changes in the histological structure of exocrine pancreas were observed in insulin treated animals which are reflected in more connective and fat tissue near the excretory ducts and blood vessels...en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Биолошки факултетsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173055/RS//
dc.rightsopenAccessen
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceУниверзитет у Београдуsr
dc.subjectinsulinsr
dc.subjectinsulinen
dc.subjectpankreassr
dc.subjectamilazasr
dc.subjectzimogene granulesr
dc.subjectacinusne ćelije, fraktalna analizasr
dc.subjectstereologijasr
dc.subjectbinukleusne ćelijesr
dc.subjectpancreasen
dc.subjectamylaseen
dc.subjectzymogene granulesen
dc.subjectacinar cellsen
dc.subjectfractal analysisen
dc.subjectstereologyen
dc.subjectbinuclear cellsen
dc.titleMorfološke promene egzokrinog pankreasa pacova izazvane insulinomsr
dc.title.alternativeInsulin-induced morphological changes in the exocrine pancreas of raten
dc.typePhD thesis
dc.rights.licenseBY-NC-ND
dc.identifier.fulltexthttps://nardus.mpn.gov.rs/bitstream/id/69843/Disertacija.pdf
dc.identifier.fulltexthttps://nardus.mpn.gov.rs/bitstream/id/69844/IzvestajKomisije28442.pdf
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_nardus_18066


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

openAccess
Except where otherwise noted, this item's license is described as openAccess