Morfološke promene egzokrinog pankreasa pacova izazvane insulinom
Insulin-induced morphological changes in the exocrine pancreas of rat
Метаподаци
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Egzokrini i endokrini pankreas ostvaruju složenu anatomsku i funkcijsku
interakciju. Naime, egzokrini deo pankreasa je pod uticajem hormona endokrinog, ne
samo sistemski kao ostale ćelije organizma, već i direktno, preko veze portnog sistema
acinusa i Langerhansovog ostrvca. Pokazano je da i endogeni i egzogeni insulin
izazivaju povećanu sintezu pankreasnih enzima u acinusima, a time i njihov rast. Veza
strukturnog (ćelijskog/tkivnog) i funkcijskog odgovora egzokrinog pankreasa na
periportalni nivo insulina ukazuje na visok i kompleksan stepen insulo-acinusne
interakcije u fiziološkim, a pogotovo u patološkim stanjima.
Cilj ove doktorske disertacije je da istraži histološke promene egzokrinog
pankreasa i ultrastrukturne promene acinusnih ćelija tokom remodeliranja izazvanog
insulinom.
U eksperimentu su korišćeni Wistar pacovi, starosti dva meseca. Pacovi su
podeljeni u šest grupa, četiri grupe su primale i.p. nisku (0.4 IU) ili visoku (4 IU) dozu
insulina, jedan (akutno) ili tri dana (...hronično). Preostale dve grupe na isti način primale
su fiziološki rastvor i predstavljale su odgovarajuće kontrole. Tri sata nakon poslednjeg
tretmana, sve životinje su žrtvovane dekapitacijom. Odmah nakon žrtvovanja, pankreas
je izolovan, slikan, izmeren i odvojen za svetlosnui elektronsku mikroskopiju. Efekti
insulina na histološke promene egzokrinog pankreasa analizirane su svetlosnom
mikroskopijom (hematoksilin/eozin bojenje, AZAN), fraktalnom analizom,
imunohistohemijom (amilaza, pHH3 – fosforilisana forma histona H3 (eng.
PhosphoHistone H3), Ki-67 i PCNA – nukleusni antigen proliferišućih ćelija (eng.
Proliferating Cell Nuclear Antigen)) i elektronskom mikroskopijom (volumenska
gustina citoplazme, dijametar i volumenska gustina zimogenih granula, broj i
volumenska gustina nukleusa)...
Exocrine and endocrine pancreas achieves complex anatomical and functional
interaction. Namely, the exocrine part of the pancreas is influenced by the endocrine
hormone, not only systemically as other cells in organizm, but also directly, via the islet
acinar portal vascular system. It has been shown that both endogenous and exogenous
insulin cause an increased synthesis of pancreatic enzymes in the acinus and hence their
growth. The relationship between the structural (cellular/tissue) and functional response
of exocrine pancreas to the periportal level of insulin indicates a high and complex level
of insulin-acinus interaction in the physiological and especially in the pathological state.
The goal of this doctoral dissertation was to investigate the histological
changes of exocrine pancreas and ultrastructural changes of acinar cells during insulininduced
remodeling.
Two-month-old male Wistar rats were used. Rats were divided into six groups,
four groups were treated i.p. with low (0....4 IU) or high (4 IU) dose of insulin, one
(acutely) or three (chronically) days. The other two groups, in the same way, were
received 0.9% saline solution and served as a corresponding control. Three hours after
the last injection, the animals were sacrificed by decapitation. Immediately after
sacrificing, the pancreas was isolated, pictured, weighted and prepared for light and
electron microscopy. The effect of insulin on the histological changes of exocrine
pancreas was analyzed by light microscopy (hematoxylin/eosin staining, AZAN), fractal
analysis, immunohistochemistry (amylase, pHH3, Ki67 and PCNA) and electron
microscopy (volume density of cytoplasm, diameter and volume density of zymogene
granules, as well as number and volume density of nuclei).
Discrete changes in the histological structure of exocrine pancreas were
observed in insulin treated animals which are reflected in more connective and fat tissue
near the excretory ducts and blood vessels...