Fitohemijski skrining i procena antioksidantnog i antiinflamatornog potencijala sekundarnih biomolekula u vrstama roda Plantago L.

Beara (Krstić), Ivana

Phytochemical screening and evaluation of antioxidant and anti-inflammatory potential of secondary metabolites of Plantago L. species

Abstract

Karakterizacija metanolnih ekstrakata jedanaest vrsta samoniklih bokvica (rod Plantago L.) obuhvatala je fitohemijski skrining i ispitivanje antioksidantne i antiinflamatorne aktivnosti. Primenom LC-MS/MS tehnike odreĊen je sadržaj odabranih sekundarnih biomolekula. Antioksidantna aktivnosti ekstrakata (sposobnost neutralizacije slobodnih radikala, redukcioni potencijal i inhibicija lipidne peroksidacije) ispitana je primenom spektrofotometrijskih metoda. U cilju odreĊivanja antiinflamatornog potencijala, razvijena je in vitro metoda za praćenje aktivnosti trombocitne ciklooksigenaze-1 i 12-lipoksigenaze. Svi ispitani ekstrakti pokazali su znaĉajnu biološku aktivnost.

Characterization of methanol extracts of eleven Plantago L. species included phytochemical screening and evaluation of antioxidant and anti-inflammatory activity. The content of several secondary metabolites was determined by LC-MS/MS technique. Antioxidant activity of extracts (radical scavenger capacity, reduction potential and inhibition of lipid peroxidation) was examined by spectrophotometric methods. With the intention to evaluate anti-inflammatory activity, an in vitro method was developed to measure activity of platelet cyclooxygenase-1 and 12-lipoxygenase. All examined extracts showed noticeable biological activity.

Faculty:
University of Novi Sad, Faculty of Science
Date:
09-07-2010

Keywords:

Plantago L. / Plantago L. / antioksidantna aktivnost / antiinflamatorna aktivnost / LC-MS/MS / ciklooksigenaza-1 / 12-lipooksigenaza / antioxidant activity / anti-inflammatory activity / LC-MS/MS analysis / cyclooxygenase-1 / 12-lipooxygenase

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