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Biohemijska i fiziološka karakterizacija klonovatopole (Populus spp.) u procesu fitoekstrakcije bakra, nikla i kadmijuma

Biohemical and physiological characterization of three poplar clones (Populus spp.) during the copper, nickel and cadmium phytoextraction process

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Author
Kebert, Marko
Mentor
Mimica-Dukić, Neda
Štajner, Dubravka
Committee members
Malenčić, Djordje
Orlović, Saša
Orčić, Dejan
Mimica-Dukić, Neda
Štajner, Dubravka
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Abstract
Predmet ovog istaživanja  bio je ispitivanje uticaja jona tri teška metala (Ni2+, Cu2+ i Cd2+) u dve toksične koncentracije u zemljištu na fiziološke i biohemijske karakteristike  odabranih klonova topola, M1, B229 i Pe 19/66. Ispitan je i potencijal odabranih klonova topola da vrše fitoekstrakciju-akumulaciju teških metala iz zemljišta u svoje nadzemne delove što dovodi  do dugoročnog uklanjanja ovih perzistentnih polutanata iz životne sredine. Takođe, ispitan je uticaj teških metala na antioksidantni potencijal, sposobnost  neutalizacije slobodnih radikala, aktivnosti antioksidantih enzima kao i  na sadržaj slobodnih i konjugovanih poliamina (Put, Spm, Spd),  određenih HPLC analizom,  i sadržaj biljnih hormona poput indol-3-sirćetne kiseline i  abscisinske  kiseline,  određenih GC/MS analizom, u listovima i korenovima klonova topola.  
The aim of this study was to estimate and compare phytoextraction capacities of three poplar clones (M1, B229 and Pe 19/66) in soil. Furthermore, the goal was to assess  different biological responses among the poplar clones during exposure to different concentration of three heavy metal ions (Ni2+, Cu2+ i Cd2+). In order to track changes in  poplars’mineral, physiological, biochemical and antioxidant status during the  abiotic stress, quantification of physiological properties, free and conjugated polyamines, total phenolics  as well as quantification of phytohormones (indol-3-acetic and abscisic acid) was done. Furthermore, assessment of antioxidant potential by tracking radical scavenger capacities (RSC) against DPPH, ABTS, OH and NO radicals and by measuring enzymes activities (SOD, GSH-Px, GPx, GR) in vitro was performed in root and leaves of poplar clones.
Faculty:
Универзитет у Новом Саду, Природно-математички факултет
Date:
12-12-2014
Keywords:
reaktivne kiseonične vrste / Poplars / abscisinska kiselina (ABA) / indol-3 sirćetna kiselina (IAA) / antioksidantna aktivnost / poliamini / AAS / HPLC / GC/MSlizom / u listovima i korenovima klonova topola. / phytoextraction / heavy metals / reactive oxygen species / abscisic acid (ABA) / indol-3 acetic acid (IAA) / antioxidant activity / polyamines / AAS / HPLC / GC-MS
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Handle
https://hdl.handle.net/21.15107/rcub_nardus_1768
URI
https://nardus.mpn.gov.rs/handle/123456789/1768
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