Приказ основних података о дисертацији
Određivanje fenolnih jedinjenja u plodovima nekih biljaka iz familije Rosaceae i antioksidativne aktivnosti njihovih sintetičkih derivata
| dc.contributor.advisor | Joksović, Ljubinka | |
| dc.contributor.other | Ćirić, Andrija | |
| dc.contributor.other | Šmit, Biljana | |
| dc.contributor.other | Kreft, Samo | |
| dc.creator | Mihailović, Nevena | |
| dc.date.accessioned | 2020-10-24T12:31:00Z | |
| dc.date.available | 2020-10-24T12:31:00Z | |
| dc.date.issued | 2019-09-20 | |
| dc.identifier.uri | http://eteze.kg.ac.rs/application/showtheses?thesesId=7531 | |
| dc.identifier.uri | https://fedorakg.kg.ac.rs/fedora/get/o:1252/bdef:Content/download | |
| dc.identifier.uri | https://nardus.mpn.gov.rs/handle/123456789/17536 | |
| dc.description | U poslednjih nekoliko decenija, interes za izučavanjem poznatih i pronalaženjem novih vrsta koje mogu imati značajan antioksidativni potencijal sve više raste. Imajući u vidu da su fenolna jedinjenja poznata kao jako dobri antioksidansi, a da su glavni izvori ovih jedinjenja biljke, konstantno se izučavaju biljne vrste koje potencijalno poseduju visok sadržaj prirodnih antioksidanasa. Familija Rosaceae obuhvata veliki broj biljnih vrsta koje ljudi od davnina koriste u svojoj ishrani, ali su mnoge divlje vrste nedovoljno proučavane, pa je deo ove disertacije baziran na analizi fenolnih jedinjenja u divljim plodovima jabuke, maline i trešnje. U cilju dobijanja maksimalnog prinosa ovih jedinjenja iz biljnog materijala, izvršena je optimizacija metode ekstrakcije fenolnih jedinjenja, i definisani su optimalni uslovi za ekstrakciju, koji obuhvataju pravilan odabir rastvarača (i njegove koncentracije), odnos mase uzorka i zapremine rastvarača, vreme i temperaturu ekstrakcije. U ovako dobijenim ekstraktima, analiziran je ukupan sadržaj fenolnih jedinjenja (ukupnih fenola, fenolnih kiselina, flavonoida i antocijanina), a rezultati su omogućili određivanje optimalnih uslova ekstrakcije ovih jedinjenja u uzorcima primenom softvera Design-Expert® i metodologije odziva površine (eng. Response Surface Methodology). Kvantifikacija pojedinačnih fenolnih jedinjenja u uzorcima dobijenim ekstrakcijom pod optimalnim uslovima izvršena je HPLC metodom, pri čemu su dobijeni rezultati potvrdili da su ovi plodovi bogati sadržajem biološki važnih fenolnih jedinjenja. Dobijene vrednosti koncentracija pojedinačnih fenolnih jedinjenja ukazale su da plodovi ispitivanih samoniklih vrsta familije Rosaceae mogu biti značajan izvor ove grupe jedinjenja u ishrani ljudi. Rezultati i metodologija analize fenolnog sastava ovih plodova, mogu biti od značaja i za buduća ispitivanja i analizu sadržaja fenolnih jedinjenja u plodovima biljaka sličnih karakteristika. Ispitana je i stabilnost i biodostupnost ovih komponenti u organizmu, simulacijom uslova in vitro Ugastrointestinalne digestije. Da bi se rasvetlio antioksidativni potencijal testiranih uzoraka, primenjen je niz metoda koje ispituju njihovu sposobnost da neutrališu štetne slobodno-radikalske vrste. Iako postoji sve veća težnja za nalaženjem i upotrebom prirodnih antioksidanasa, mogućnosti njihove univerzalne primene su često ograničene, pa se pribegava dobijanju antioksidativnih jedinjenja sintetičkim putem. U tom smislu, u ovom radu je izvršena sintetička modifikacija odabranih fenolnih kiselina, sa ciljem nalaženja novih jedinjenja sa boljim biološkim aktivnostima, pre svega antioksidativnom. Na ovaj način su dobijene dve serije jedinjenja, 1,2,4- triazol-3-tioni i 1,3,4-oksadiazoli. Dobijeni rezultati daju doprinos analizi fenolnih jedinjenja u prirodnim proizvodima, njihovoj funkcionalizaciji i primeni | sr |
| dc.description | In the past several decades, the interest in studying well-known and discovery of new species that may have significant antioxidant potential is constantly growing. Taking into account that phenolic compounds are known as good antioxidants, and that the main sources of these compounds are plants, there is a constant pursuit for plant species potentially possessing a high content of natural antioxidants. The Rosaceae family includes a large number of plant species that humans have used in their diet for a long time, but many wild species are insufficiently studied, therefore part of this PhD thesis is based on the analysis of phenolic compounds in wild apples, raspberries, and cherries (family Rosaceae). In order to obtain the maximum yield of these compounds, optimization of the extraction method of phenolic compounds from plant material was performed, and optimal conditions for extraction, including the appropriate choice of the solvent (and its concentration), the solid to solvent ratio, the time and temperature of the extraction, were defined. The total content of phenolic compounds (total phenols, phenolic acids, flavonoids, and anthocyanins) was analyzed in the obtained extracts, and the obtained results were used in determination of optimal conditions for the extraction of these compounds in the samples using the DesignExpert® software and the response surface methodology. The quantification of individual phenolic compounds in the samples obtained by extraction under optimal conditions was performed by HPLC method and the results confirmed that these fruits are rich in the biologically important phenolic compounds. The obtained concentrations of individual phenolic compounds indicated that the studied wild fruit species of the Rosaceae family could be a significant source of phenolic compounds in human nutrition. The results and methodology of analyzing the phenolic composition of these fruits can be of importance for future research and analysis of phenolic compounds content in fruit or plants with similar characteristics. Furthermore, the stability and bioaccessibility of these components in the organism were examined by the simulation of the in vitro gastrointestinal digestion conditions. In order to clarify the Iantioxidant potential of the tested samples, a number of methods for determination of the ability of obtained extracts to neutralize harmful free-radical species have been applied. Although there is a growing tendency to find and use natural antioxidants, the possibilities of their universal application are often limited, therefore antioxidant compounds are often synthetically obtained. The synthetic modification of selected phenolic acids was carried out with the aim of finding a new series of compounds with better biological activities, mainly antioxidant activity. Two series of novel compounds were synthesized, 1,2,4-triazol-3-thiones and 1,3,4-oxadiazoles. The obtained results in this PhD thesis contribute to the analysis of phenolic compounds in natural products, their functionalization, and application. | en |
| dc.format | application/pdf | |
| dc.language | sr | |
| dc.publisher | Универзитет у Крагујевцу, Природно-математички факултет | sr |
| dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172016/RS// | |
| dc.rights | openAccess | en |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.source | Универзитет у Крагујевцу | sr |
| dc.title | Određivanje fenolnih jedinjenja u plodovima nekih biljaka iz familije Rosaceae i antioksidativne aktivnosti njihovih sintetičkih derivata | sr |
| dc.type | doctoralThesis | en |
| dc.rights.license | BY | |
| dc.identifier.fulltext | https://nardus.mpn.gov.rs/bitstream/id/66147/Izvestaj_Nevena_Mihailovic_PMF.pdf | |
| dc.identifier.fulltext | https://nardus.mpn.gov.rs/bitstream/id/66146/Disertacija.pdf | |
| dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_nardus_17536 |
