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Raman spectroscopy of pharmacologically active compounds and biocatalysts

dc.contributor.advisorBugarski, Branko
dc.contributor.otherKnežević-Jugović, Zorica
dc.contributor.otherBugarski, Diana
dc.contributor.otherPopović, Zoran V.
dc.creatorLazarević, Jasmina J.
dc.description.abstractU ovoj disertaciji Ramanova spektroskopija korišćena je za sveobuhvatnu analizu primarnih mezenhimskih matičnih ćelija, poreklom iz periodoncijuma, mekog vezivnog tkiva koje spaja koren zuba za alveolarnu kost i ima ulogu u održavanju mineralizacije i ublažavaanja sila koje se javljaju ž
dc.description.abstractIn this dissertation, the utilization of Raman spectroscopy for comprehensive analysis of primary mesenchymal stem cells from periodontal ligament, a soft connective tissue which anchors tooth root for the alveolar bone, was demonstrated. Since every cell has the unique Raman spectrum, Raman spectroscopy was used with the purpose to gain the insight in intrinsic biochemical prole on a single cell level. Mesenchymal stem cells from periodontal ligament were stimulated to dierentiate towards osteogenic, chondrogenic, and adipogenic lineages. These dierentiated cells were investigated by means of Raman spectroscopy as well. Microscopic glass cover-slips were used as substrates due to its cost-eectiveness and common use in laboratories, which is important for its clinical application. On the other hand, glass has strong Raman response, but it was demonstrated that the analysis was possible by taking into account certain spectral region in which its inuence was eliminated. In all sample groups, the same type of behavior was noticed, manifested as the increase of relative intensity Raman band of proteins/lipids, and the decrease of relative intensity of nucleic acids Raman bands. Further, the extensive statistical analysis in the form of principal component analysis was performed, revealing signi- cant groupings of cells with the same spectral features, which conrmed previously obtained results. Despite the heterogeneity of primary mesenchymal stem cells and their dierentiated lineages, it was proved that micro-Raman spectroscopy could adequately distinguish cell status, which is important for its clinical and medical use...en
dc.publisherУниверзитет у Београду, Технолошко-металуршки факултетsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45018/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.sourceУниверзитет у Београдуsr
dc.subjectRamanova spektroskopijasr
dc.subjectRaman spectroscopyen
dc.subjectmezehnimske matične ćelijesr
dc.subjectanaliza pojedinačnih ćelijasr
dc.subjectmeđumolekularne vibracijesr
dc.subjectMesenchymal stem cellsen
dc.subjectSingle cell analysisen
dc.subjectIntermolecular vibrationsen
dc.titleРаманова спектроскопија фармаколошки активних супстанци и биокатализатораsr
dc.title.alternativeRaman spectroscopy of pharmacologically active compounds and biocatalystsen

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