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Раманова спектроскопија фармаколошки активних супстанци и биокатализатора

Raman spectroscopy of pharmacologically active compounds and biocatalysts

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Disertacija.pdf (18.66Mb)
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Author
Lazarević, Jasmina J.
Mentor
Bugarski, Branko
Committee members
Knežević-Jugović, Zorica
Bugarski, Diana
Popović, Zoran V.
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Abstract
U ovoj disertaciji Ramanova spektroskopija korišćena je za sveobuhvatnu analizu primarnih mezenhimskih matičnih ćelija, poreklom iz periodoncijuma, mekog vezivnog tkiva koje spaja koren zuba za alveolarnu kost i ima ulogu u održavanju mineralizacije i ublažavaanja sila koje se javljaju žvakanjem...
In this dissertation, the utilization of Raman spectroscopy for comprehensive analysis of primary mesenchymal stem cells from periodontal ligament, a soft connective tissue which anchors tooth root for the alveolar bone, was demonstrated. Since every cell has the unique Raman spectrum, Raman spectroscopy was used with the purpose to gain the insight in intrinsic biochemical prole on a single cell level. Mesenchymal stem cells from periodontal ligament were stimulated to dierentiate towards osteogenic, chondrogenic, and adipogenic lineages. These dierentiated cells were investigated by means of Raman spectroscopy as well. Microscopic glass cover-slips were used as substrates due to its cost-eectiveness and common use in laboratories, which is important for its clinical application. On the other hand, glass has strong Raman response, but it was demonstrated that the analysis was possible by taking into account certain spectral region in which its inuence was eliminated. In all sample gro...ups, the same type of behavior was noticed, manifested as the increase of relative intensity Raman band of proteins/lipids, and the decrease of relative intensity of nucleic acids Raman bands. Further, the extensive statistical analysis in the form of principal component analysis was performed, revealing signi- cant groupings of cells with the same spectral features, which conrmed previously obtained results. Despite the heterogeneity of primary mesenchymal stem cells and their dierentiated lineages, it was proved that micro-Raman spectroscopy could adequately distinguish cell status, which is important for its clinical and medical use...

Faculty:
Универзитет у Београду, Технолошко-металуршки факултет
Date:
26-06-2020
Projects:
  • info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45018/RS// (RS-45018)
  • info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS// (RS-46010)
Keywords:
Ramanova spektroskopija / Raman spectroscopy / mezehnimske matične ćelije / analiza pojedinačnih ćelija / polimorfizam / međumolekularne vibracije / Mesenchymal stem cells / Single cell analysis / Polymorphism / Intermolecular vibrations
[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_nardus_17463
URI
http://eteze.bg.ac.rs/application/showtheses?thesesId=7605
https://fedorabg.bg.ac.rs/fedora/get/o:22551/bdef:Content/download
http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=20693769
https://nardus.mpn.gov.rs/handle/123456789/17463

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