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Prilog određivanju fumonizina u žitaricama i lekovitom bilju u Srbiji

A contribution to the determination of fumonisins in grain and medicinal plants in Serbia

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Author
Jakšić, Sandra
Mentor
Abramović, Biljana
Committee members
Jovanović, Ljiljana
Abramović, Biljana
Mašić, Zoran
Jajić, Igor
Živkov-Baloš, Milica
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Abstract
Ispitana  je  mogućnost ekstrakcije fumonizina iz kukuruza sa vodom i fosfatnim puferom, umesto sa smešom acetonitril–metanol–voda.  Ekstrakcione metode neorganskim  rastvaraĉima  su  uspešno  primenjene za odreĊivanje  fumonizina  B1, B2 i  B3 metodom  tečne hromatografije sa fluorescentnom detekcijom, kao i ukupnih fumonizina pomoću  imunohemijske metode. Analiziran  je  veći broj  uzoraka na  prisustvo i sadržaj fumonizina.  Ispitani su uzorci  kukuruza (235), sakupljeni tokom višegodišnjeg perioda (2005.  i 2009−2013.  godine)  i pšenice (83) roda 2010. i 2012. godine, sa područja  severne Srbije.  Ispitana je kontaminiranost kukuruza sa područja  severne Srbije fumonizinima, drugim mikotoksinima i plesnima, kao i mogući uticaj klimatskih faktora na stepen kontaminacije.  Rezultati kontaminiranosti useva fumonizinima  za svaku godinu  pojedinačno  su povezivani sa vremenskim prilikama koje su  pratile istu. Različita hrana na bazi žitarica je analizirana ELISA metodama. Razvijena j...e ELISA i metoda  tečne hromatografije sa fluorescentnom detekcijom  za određivanje fumonizina u tri vrste lekovitog bilja sa područja Srbije.  Ispitana je mogućnost  primene infracrvene spektroskopije sa Furijeovim transformom  za  odredivanje  fumonizina. Razvijen je  ekspertni sistem za rešavanje problema izbora  optimalnog postupka određivanja fumonizina u kukuruzu.

Possibility of fumonisin extraction from maize using water and phosphate buffer instead of acetonitrile-methanol-water mixture was examined. The methods of extraction without organic solvents have successfully been applied for the determination of fumonisins  B1, B2 and  B3 using  liquid chromatography-fluorescence detection  method,  as well as total fumonisins using enzyme-linked immunosorbent  assay  (ELISA).  Large number of samples was analyzed for the presence and content of fumonisins. Maize samples (total 235) collected throughout several-year period (2005 and 2009-2013) and wheat samples (total 83) from 2010 and 2012 harvest originating from the territory of northern Serbia were analyzed. Contamination of maize originating from the territory of northern Serbia with fumonisins and other mycotoxins and moulds was examined, as well as the potential impact of climatic factors on contamination level.  The results on the fumonisin-contamination of cereals obtained for each individua...l year are related with the climatic  conditions characteristic for the relevant year. A variety of cereal-based food was analyzed using ELISA methods. Improved  liquid chromatography-fluorescence  detection method and ELISA for the determination of fumonisins in three  medicinal plant  species from the territory of Serbia were developed.  Possible application of  Fourier transform  infrared spectroscopy  (FTIR) for quantification of fumonisin was investigated.  An expert system  to solve the problem of selecting an optimal method for  determination of fumonisins in  maize has been developed.

Faculty:
Универзитет у Новом Саду, Природно-математички факултет
Date:
02-02-2015
Projects:
  • Research on pharmacological characteristics of antimicrobial agents, introduction of new technological solutions and alternative prophylactic methods with the purpose to improve control of infectious animal disease (RS-31071)
Keywords:
fumonizini / fumonisins / tečna hromatografija / ELISA / ekstrakcija / žitarice / lekovito bilje / FTIR / ekspertni sistem / HPLC / ELISA / extraction / grain / medicinal plants / FTIR / expert system
[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_nardus_1737
URI
https://nardus.mpn.gov.rs/handle/123456789/1737
http://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija142771673240715.pdf?controlNumber=(BISIS)91951&fileName=142771673240715.pdf&id=3427&source=NaRDuS&language=sr
http://www.cris.uns.ac.rs/record.jsf?recordId=91951&source=NaRDuS&language=sr
http://www.cris.uns.ac.rs/DownloadFileServlet/IzvestajKomisije141701731621719.pdf?controlNumber=(BISIS)91951&fileName=141701731621719.pdf&id=3054&source=NaRDuS&language=sr

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