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Assessment of the antioxidant and anti-inflammatory potential of vitamin B1 in Japanese quails (Coturnix japonica) subacute treated with chlorpyrifos

dc.contributor.advisorBorozan, Sunčica
dc.contributor.advisorPrevendar Crnić, Andreja
dc.contributor.otherIvanović, Saša
dc.contributor.otherDacić, Sanja
dc.contributor.otherPeković, Sanja
dc.creatorĆupić Miladinović, Dejana
dc.date.accessioned2020-08-12T13:56:21Z
dc.date.available2020-08-12T13:56:21Z
dc.date.issued2019-02-18
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=7502
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:22390/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=17119241
dc.identifier.urihttps://nardus.mpn.gov.rs/handle/123456789/17337
dc.description.abstractHlorpirifos (CPF) je hlorovani organofosfatni pesticid, odnosno insekticid, koji se pre svega koristi u poljoprivredi. Ovaj insekticid svoje delovanje kod sisara ostvaruje na najmanje tri načina: inhibicijom aktivnosti enzima acetilholinesteraze (AChE), izazivanjem oksidativnog stresa i izazivanjem funkcionalnog poremećaja endokrinih žlezda. Vitamin B1 (tiamin) spada u grupu hidrosolubilnih vitamina, koji se ne sintetiše u organizmu sisara, pa se mora unositi hranom. Tiamin preko acetil koenzima A (acetil-CoA) učestvuje u sintezi AChE i njegov nedostatak potencira delovanje organofostata. Postoji zajednička veza između deficita tiamina, neurodegenerativnih promena, oksidativnog stresa i apoptoze. Imajući u vidu da u dosadašnjoj literaturi nema podataka o ispitivanju protektivnog delovanja B1 vitamina nakon trovanja CPF-om kod ptica, postoji realna potreba da se ispita njegovo dejstvo u odgovarajućem modelu. Cilj ovog istraživanja je bio da se ispita uticaj vitamina B1 u plazmi, jetri i mozgu kod Japanskih prepelica (Coturnix japonica) koje su bile tretirane različitim dozama CPF-a. Praćeni su sledeći parametri oksidativnog stresa: koncentracija malondialdehida (MDA), koncentracija nitrita (NO2–), koncentracija redukovanog glutationa (GSH), aktivnost katalaze (CAT), superoksid dismutaze (SOD), glutation-S-transferaze (GST), mijeloperoksidaze (MPO), arilesteraze (ARE); ali i aktivnost acetil i butirilholinesteraze (AChE i BuChE), kao i aktivnost inducibilne azot-oksid sintaze (iNOS), ciklooksigenaze (COX), ekstracelularno regulisane kinaze (ERK) (regulatora apoptoze), c-jun regulisane kinaze (JNK), nivo B-ćelijskog limfoma (Bcl-2). Kod prepelica koje su bile tretirane CPF-om, ispitivan je i uticaj vitamina B1 u plazmi i mozgu, na produkciju citokina – interleukina 1 (IL-1) i interleukina 6 (IL-6), kao i antioksidativni kapacitet samog vitamina B1. Ispitivanje je rađeno na osamdeset Japanskih prepelica (2 kontrolne grupe i 6 eksperimentalnih, n=10/po grupi) muškog pola, starosti 3-4 nedelje...sr
dc.description.abstractChlorpyrifos (CPF) is a chlorinated organophosphate pesticide, or insecticide, which is primarily used in agriculture. This insecticide achieves its function in the mammals in at least three ways: by inhibiting acetylcholinesterase (AChE), causing oxidative stress and functional disorder of the endocrine glands. Vitamin B1 (thiamine) belongs to a group of hydrosoluble vitamins, which is not synthesized in the mammalian organism, and must be taken with food. Thiamine, through acetyl Coenzyme A (acetyl-CoA), participates in the synthesis of AChE, and its deficiency potentiates the effects of organophosphates. There is a common link between thiamine deficiency, neurodegenerative changes, oxidative stress, and apoptosis. Bearing in mind that in the current literature there is no data on the testing of the protective activity of B1 vitamin after CPF poisoning in birds, there is an unmet need to examine its effects in the appropriate model. The aim of this study was to investigate the influence of vitamin B1 on biochemical changes in the plasma, liver and brain of Japanese quail (Coturnix japonica) treated with different doses of chlorpyrifos. The following parameters of oxidative stress were examined: concentration of malondialdehyde (MDA), nitrite concentration (NO2-), concentration of reduced glutathione (GSH), activity of catalase (CAT), superoksid dismutase (SOD) glutathione-S-transferase (GST), myeloperoxidase (MPO), arylesterase (ARE). Cholinesterase activity (acetylcholinesterase – AChE and butyrylcholinesterase –BuChE), activity of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX2) and extracellular signal–regulated kinase (ERK) (apoptotic regulators), c-jun regulated kinase (JNK), and level of B-cell lymphoma (Bcl-2), were followed as well. In the plasma and brain of quails treated with CPF, we also studied the influence of vitamin B1 on the production of cytokines-interleukin 1 (IL-1) and interleukin 6 (IL-6), as well as the antioxidative capacity of vitamin B1. The study was conducted on eighty male Japanese quails (2 control groups and 6 experimental groups, n = 10 per group), 3-4 weeks old...en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Факултет ветеринарске медицинеsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41014/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46009/RS//
dc.rightsopenAccessen
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceУниверзитет у Београдуsr
dc.subjecthlorpirifossr
dc.subjectchlorpyrifosen
dc.subjecttiaminsr
dc.subjectJapanske prepelicesr
dc.subjectinflamacijasr
dc.subjectoksidativni stressr
dc.subjectthiamineen
dc.subjectJapanese quailsen
dc.subjectinflammationen
dc.subjectoxidative stressen
dc.titleIspitivanje antioksidativnog i antiinflamatornog potencijala vitamina B1 kod japanskih prepelica (Coturnix japonica) subakutno tretiranih hlorpirifosomsr
dc.title.alternativeAssessment of the antioxidant and anti-inflammatory potential of vitamin B1 in Japanese quails (Coturnix japonica) subacute treated with chlorpyrifosen
dc.typedoctoralThesisen
dc.rights.licenseBY-NC-ND
dc.identifier.fulltexthttps://nardus.mpn.gov.rs/bitstream/id/64818/Disertacija.pdf
dc.identifier.fulltexthttps://nardus.mpn.gov.rs/bitstream/id/64819/IzvestajKomisije22989.pdf
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_nardus_17337


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