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The impact of genetic variants of the enzyme fatty acid metabolism on their effect in patients with rheumatoid arthritis

dc.contributor.advisorTomić-Lučić, Aleksandra
dc.contributor.otherVeselinović, Mirjana
dc.contributor.otherPavlović, Sonja
dc.contributor.otherPetronijević, Milan
dc.creatorTomić-Smiljanić, Marijana
dc.date.accessioned2020-02-26T15:44:58Z
dc.date.available2020-02-26T15:44:58Z
dc.date.available2020-07-03T15:19:41Z
dc.date.issued2019-12-24
dc.identifier.urihttp://eteze.kg.ac.rs/application/showtheses?thesesId=7263
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/12020
dc.identifier.urihttps://fedorakg.kg.ac.rs/fedora/get/o:1233/bdef:Content/download
dc.description.abstractUvod: Reumatoidni artritis (RA) karakteriše hronično zapaljenje, razaranje zglobova i povećan rizik za kardiovaskularna oboljevanja Unos hranom omega-3 i omega-6 polinezasićenih masnih kiselina utiče na inflamaciju i kardiovaskularni rizik.Genetske varijacije utiču na način na koji telo odgovara na neke nutrijente i sastojke hrane, i koje materije se mogu razgraditi i ispravno iskoristiti. Cilj:Proceniti uloge genetičkih varijanti u genima koji kodiraju enzime u metaboličkom putu omega-3 i omega-6 masnih kiselina u modifikaciji kliničkog efekta suplementacija sa omega-3 i omega-6 masnim kiselinama, i uticaj suplementacija sa omega-3 i omega-6 masnim kiselinama na aktivnost bolesti, oksidativni stres, endotelnu funkciju i hemostazu kod pacijenata sa RA. Materijal i metode: 60 pacijentkinja sa RA je podeljeno u prvu grupu (20 pacijenata) koji su uzimali 5 gel kapsula Omega 3Kardio®u toku 3 meseca, drugu grupu (20 pacijenata) koji su uzimali po 3 gel kapsula Omega 3Kardio® i dve gel kapsule ulja noćurka u toku 3 meseca i treću, kontrolnu grupu (20 pacijenata) koji su nisu uzimali suplementaciju. Rađena je analiza genetičkih varijanti u genima koji kodiraju enzime metabolizma puta omega-3 i omega-6 masnih kiselina: rs174556, rs174561, rs3834458, rs174570 i rs968567 po metodologiji zasnovanoj na PCR-ukao i skvenciranje po Sangeruabiohemijske i hematološke analize su urađene u laboratorijima Kliničkog centra Kragujevac.Koncenracija masnih kiselina u krvi je urađena pre i posle suplementacije metodom gasne hromatografije.Redoks status je određivan spektrofotometrijski. Rezultati:Prisustvo određenih genetičkih varijanti gena za enzime desaturaze masnih kiselina kod pacijenati sa RAkoji su uzimali suplementaciju omega 3 i omega 6 masnim kiselinama u toku 3 meseca utiču na koncentraciju arahidonske kiseline idokozaheksaenoinska kiselina u fosfolipidima eritrocita, kao i na smanjenje parametara inflamacije u odnosu na kontrolnu grupu.Suplementacija omega3 i omega 6masnim kiselinama redukuje agregaciju trombocita kod pacijenta sa RA.Prooksidacioni i antioksidacioni parametri su u grupama pacijenata sa RA koje su uzimale suplementaciju omega 3 i omega 6 masnim kiselinama pokazali smanjenje oksidacionog stresa i povećanje aktivnosti antioksidacionog sistema zaštite. Suplementacija omega 3 i omega 6 masnim kiselinama kod pacijenata sa RA utiče na smanjenje aktivnosti bolesti. Zaključak:Kod pacijenata sa RA koji su uzimali suplementaciju omega 3 i omega 6 masnim kiselinama dolazi do smanjenja aktivnosti bolesti, parametara inflamacije, hemostaze i oksidativnog stresa. Genomske informacije donose bolje razumevanje interakcija gena i nutijenata, a s krajnjim ciljem razvoja personaliziranih smernica za pravilnu ishranu koja može doprineti lečenju reumatoidnog artritisa.sr
dc.description.abstractIntroduction: Rheumatoid arthritis (RA) is characterized by chronic inflammation, joint destruction and an increased risk for cardiovascular morbidity dietary intakes of omega-3 and omega-6 polyunsaturated fatty acids affects inflammation and cardiovascular risk. Genetic variations affect the way the body responds to certain nutrients and food components, and materials that can break down and properly utilized. Aim:To assess the role of genetic variants in genes encoding enzymes in the metabolic pathway of omega-3 and omega-6 fatty acids in the modification of the clinical effect of supplementation with omega-3 and omega-6 fatty acids, and the effect of supplementation with omega-3 and omega-6 fatty acids on the activity of the disease, oxidative stress, endothelial function and hemostasis in patients with RA Material and methods: 60 patients with RA is divided into the first group (20 patients), to which 5 of gel capsules Omega 3 Kardio® for 3 months, the second group (20 patients) who have taken after 3 of gel capsules Omega 3 Kardio® and two gel capsules oil evening primrose oil for 3 months, and the third, control group (20 patients) who were taking no supplements. Performed the analysis of genetic variants in genes encoding the metabolic enzyme times omega- 3 and omega-6 fatty acids: rs174556, rs174561, rs3834458, rs174570 and rs968567 according to a methodology based on the PCR as well as skvenciranje by Sanger and the biochemical and haematological analyzes were performed in laboratories of Clinical center Kragujevac. Is the concentration of fatty acids in blood was performed before and after the supplementation of the GC method. Redox status was determined by spectrophotometry Results: The presence of specific genetic variants of the gene for the enzyme fatty acid desaturases in RA patients who received supplementation with omega-3 and omega-6 fatty acids for 3 months, affecting the concentration of arachidonic acid and docosahexaenoic acid in the erythrocyte phospholipids, as well as the reduction of the parameters of inflammation in relation to control group. Supplementation of omega 3 and omega 6 fatty acids reduces platelet aggregation in a patient with RA. Prooxidant and antioxidant actions are parameters in the groups of patients with RA who received supplementation with omega-3 and omega-6 fatty acidsshowed a decrease in oxidative stress and the increase in activity of the antioxidant protection system. Supplementation of omega 3 and omega 6 fatty acids in patients with RA affects the reduction in disease activity. Conclusions: The presence of specific genetic variants of the gene for the enzymes desaturase in patients with RA who received supplementation with omega-3 and omega-6 fatty acids, a decrease in disease activity parameters of inflammation, hemostasis and oxidative stress. Genomic information yields better understanding of the interaction of genes and of nutrient, and with the ultimate goal of developing personalized guidelines for proper nutrition that can contribute to the treatment of rheumatoid arthritis. disease.en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Крагујевцу, Факултет медицинских наукаsr
dc.rightsopenAccessen
dc.sourceУниверзитет у Крагујевцуsr
dc.subjectreumatoidni artritissr
dc.subjectrheumatoid arthritisen
dc.subjectоmega-3/omega-6 fatty acidsen
dc.subjectnutrigeneticen
dc.subjectsoxidative stressen
dc.subjectomega 3/omega 6 masne kiselinesr
dc.subjectnutrigenetikasr
dc.subjectoksidacioni stressr
dc.titleUticaj genskih varijanti enzima metabolizma masnih kiselina kod pacijenata obolelih od reumatoidnog artritisasr
dc.title.alternativeThe impact of genetic variants of the enzyme fatty acid metabolism on their effect in patients with rheumatoid arthritisen
dc.typedoctoralThesisen
dc.rights.licenseBY-NC-ND
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/49959/Marijana_Tomic_Smiljanic_Medicinski.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/49958/Disertacija.pdf


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