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Immunohistochemical characteristics and redox state of the pituitary-gonadal system after administration of anabolic steroid to peripubertal and adult rats

dc.contributor.advisorMilosavljević, Zoran
dc.contributor.otherPantović, Suzana
dc.contributor.otherJakovljević, Vladimir
dc.contributor.otherMilošević, Verica
dc.creatorSretenović, Jasmina Z.
dc.date.accessioned2020-02-26T15:44:47Z
dc.date.available2020-02-26T15:44:47Z
dc.date.available2020-07-03T15:16:33Z
dc.date.issued2019-09-27
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/12006
dc.identifier.urihttp://eteze.kg.ac.rs/application/showtheses?thesesId=7165
dc.identifier.urihttps://fedorakg.kg.ac.rs/fedora/get/o:1215/bdef:Content/download
dc.description.abstractSažetak: Uvod: Anabolički androgeni steroidi (AAS) predstavljaju sintetske derivate muškog polnog hormona testosterona. Tokom poslednjih nekoliko decenija, zloubotreba AAS postala je široko rasprostranjena među profesionalnim ali i među rekreativnim sportistima. Poslednjih godina, nandrolon dekanoat predstavlja jedan od najčešće zloupotrebljavanih AAS u sportu. Cilj: Cilj ovog istraživanja bio je da se ispitaju efekti hronične primene nandrolon dekanoata i treninga plivanjem na histomorfometrijske parametre gonadotropnih ćelija hipofize, histološke promene na testistu, skeletnom mišiću i masnom tkivu, kao i na redoks status kod peripubertalnih i adultnih mužjaka pacova. Materijal i metode: U studiju su uključene 64 životinje muškog pola (po 32 životinje za svaku starosnu grupu) starosti 5 nedelja (peripubertalni) i starosti 10 nedelja (adultni) svrstanih u 4 ispitivane grupe: kontrolna grupa - (T-N-), grupa koja prima steroide (T-N+), grupa koja trenira plivanje (T+N-) i grupa koja trenira plivanje i prima steroide (T+N+). Životinje koje su primale steroide dobijale su jednom nedeljno nandrolon dekanoat u dozi od 20 mg/kg telesne mase, subkutano. Životinje koje su trenirale plivanje, svakog dana su plivale 1sat /5 dana u nedelji. Nakon završenog četvoronedeljnog eksperimentalnog procesa, životinje su žrtvovane. Izolovana tkiva hipofize, testisa, skeletnog mišića i masnog tkiva rutinski su obrađivana i pripremljena prema standardnim histološkim protokolima. U prikupljenim uzorcima krvi, određivane su vrednosti testosterona, LH, FSH, leptina, insulina kao i prooskidativni markeri i enzimi sistema antioksidativne zaštite. Rezultati: Naši rezultati su pokazali da tretman nandrolon dekanoatom značajno smanjuje volumensku gustinu gonadotropnih ćelija hipofize u obe ispitivane starosne populacije. Broj ćelija LH ćelija hipofize na mm2 značajno je smanjen nakon samostalne i udružene primene nandrolona i treninga plivanjem u adultnoj populaciji mužjaka. Serumske vrednosti LH i FSH hormona snižene su nakon tretmana nandrolonom, dok su istovremeno vrednosti testosterona u krvi povišene. Ekspresija androgenog receptora u testisu smanjena je nakon tretmana nandrolonom. U obe ispitivane starosne populacije primećeno je najveće povećanje dijametra i površine skeletnog mišića nakon udružene primene nandrolon dekanoata i treninga plivanjem. U T+N+ grupi uočeno je najveće smanjenje dijametra i površine preseka adipocita kao i serumskih vrednosti leptina. Povećanje vrednosti prooksidativnih markera i u tkivu i u krvi uočeno je nakon primene nandrolona. Zaključak: Nandrolon dekanoat samostalno i u kombinaciji sa treningom plivanjem snižava histomorfometrijske parametre gonadotropnih ćelija hipofize, ekspresiju androgenog receptora u testisu, serumske vrednosti LH, FSH hormona i leptina, dok istovremeno povećava koncentraciju testosterona i dijametar skeletnih mišićnih ćelija.sr
dc.description.abstractAbstract: Introduction: Anabolic androgenic steroids (AAS) are synthetic derivatives of the male sex hormone testosterone. During the last few decades, the abuse of AAS has become widespread among professional as well as recreational sportsman. In recent years, nandrolone decanoate has been one of the most frequently abused AASs in sports. Aim: The aim of this study was to investigate the effects of the chronic administration of nandrolone decanoate and swimming training on histomorphometric parameters of the pituitary gonadotrophic cells, histological changes in the testis, skeletal muscle and fat tissue as well as redox status in peripubertal and adult male rats. Material and methods: The study included 64 male rats (32 animals per age group) 5 weeks old (peripubertal) and 10 weeks old (adults) divided into 4 experimental groups: control group - (TN-), the group that received steroids (T-N+), swimming training group (T+N-) and swimming training with steroid administration group (T+N+). The steroid receiving animals, weekly received nandrolone decanoate in a dose of 20 mg/kg b.w, subcutaneously. Rats that trained by swimming, swam every day 1 hour, 5 days a week. After completing the four-week experimental protocol, rats were sacrificed. Isolated pituitary gland, testicles, skeletal muscle and fat tissue were routinely processed and prepared according to standard histological protocols. In the collected blood samples, the values of testosterone, LH, FSH, leptin, insulin, pro-oxidative markers and enzymes of the antioxidant defense system were determined. Results: Our results have shown that the treatment with nandrolone decanoate significantly reduces the volume density of pituitary gonadotropic cells in both populations. Number of LH pituitary cells per mm2 was significantly decreased after nandrolone treatment alone or combined with swimming training in adult rats. Serum levels of LH and FSH hormone were decreased after treatment with nandrolone while levels of testosterone were increased. Expresion of androgenic receptor in testes were decreased after treatment with nandrolone. In both population the largest increase of cross-section area and diameter of skeletal muscle cells was oberved after combined administration of nandrolone and swimming training. The largesed decrease of the diameter and cross-section areas of the adipocytes and serum level of leptin was observed in T+N+ group. The increased blood and tissue levels of pro-oxidative markers was observed after treatment with nandrolone. Conclusion: Nandrolone decanoate alone or combined with swimming training decreases histomorphometric parameters of pituitary gonadotrophic cells, expresion of androgenic receptors in testes, serum levels of LH, FSH hormons and leptin. Increse of the blood testosteron levels and size of skeletal muscle cells can also be observed.en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Крагујевцу, Факултет медицинских наукаsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173009/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175043/RS//
dc.rightsopenAccessen
dc.sourceУниверзитет у Крагујевцуsr
dc.subjectnandrolonsr
dc.subjectnandroloneen
dc.subjecttreningsr
dc.subjecthipofizasr
dc.subjectskeletni mišićsr
dc.subjecttestissr
dc.subjecttrainingen
dc.subjectpituitaryen
dc.subjectskeletal muscleen
dc.subjecttestisen
dc.titleImunohistohemijske karakteristike i redoks status hipofizno-gonadalnog sistema nakon aplikacije anaboličkog steroida peripubertalnim i adultnim mužjacima pacovasr
dc.title.alternativeImmunohistochemical characteristics and redox state of the pituitary-gonadal system after administration of anabolic steroid to peripubertal and adult ratsen
dc.typedoctoralThesisen
dc.rights.licenseBY-NC-ND
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/48981/Jasmina_Sretenovic_Medicinski.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/48980/Disertacija.pdf


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