Uticaj sastojaka energetskih napitaka na funkciju miokarda i oksidativni stres u izolovanom srcu i krvi pacova
The effect of energy drink ingredients on myocardial function and oxidative stress in isolated heart and blood of rats
Author
Pušica, IrenaMentor
Đorđević, DušicaCommittee members
Živković, VladimirZdravković, Vladimir
Radovanović, Dragan
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Show full item recordAbstract
Introduction. Energy drinks (EDs) are widely used by athletes as ergogenic agents. The aims of
this study were to determine the acute, chronic and direct effects of Red Bull®, one of the most
consumed EDs, on cardiodynamics and the parameters of oxidative stress in physically active and
physically inactive rats.
Material and method. The study lasted 4 weeks. Rats were divided into 2 groups depending on
the consumption of Red Bull (3.75 ml/kg), and each group was divided into 2 subgroups,
depending on the physical activity. The trained rats were subjected to a swimming practice (1h per
day, 5 days a week). After sacrificing, the animals were sampled for venous blood to determine
the plasma redox status. Subsequently, the hearts were isolated and perfused by Langendorf
method of retrograde perfusion. The coronary flow and parameters of left ventricular function were
monitored: maximum rate of change of left ventricle pressure, minimum rate of change of left
ventricle pressure,... systolic left ventricular pressure, diastolic left ventricular pressure, and heart
rate. The biomarkers of oxidative stress in the blood and effluent were determined by the
spectrophotometric methods: superoxide anion radical, hydrogen peroxide, index of lipid
peroxidation, nitrite monoxide in the form of nitrite, as well as the parameters of antioxidant
protection system: superoxide dismutase, catalase and reduced glutathione.Results. The negative effects of the acute, chronic and direct administration of the ED on cardiac
function were registered in untrained animals. In trained animals, the acute and chronic
consumption of the ED resulted in increasing the coronary flow and most cardiodynamic
parameters, while the direct administration of the ED resulted in decreasing rates of contractility
and relaxation, and was associated with the depression of cardiac function. In untrained animals,
the administration of the ED had a pro-oxidative effect in the coronary effluent (acute and direct
administration) as well as in blood (acute and chronic administration). In trained animals, the acute,
chronic and direct administration of the ED had a pro-oxidative potential in the coronary effluent,
whereas antioxidant activity was registered in the blood after the acute administration of the ED.
Conclusion. Our study contributes to the knowledge on the effects of EDs on cardiodynamics and
the redox status correlated with the exercise status.
Faculty:
Универзитет у Крагујевцу, Факултет медицинских наукаDate:
15-11-2019Projects:
- Genetic polymorphisms of CYP genes in Serbian population (RS-MESTD-Basic Research (BR or ON)-175093)