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The influence of the expression estrogens and progesterone receptors on angiogenesis, proliferation and apoptosis of myoma cells in premenopausal and postmenopausal women

dc.contributor.advisorMitrović, Slobodanka
dc.contributor.otherŽivanović, Aleksandar
dc.contributor.otherTanasković, Irena
dc.contributor.otherRaičević, Saša
dc.creatorKljakić, Duško
dc.date.accessioned2020-02-26T15:44:42Z
dc.date.available2020-02-26T15:44:42Z
dc.date.available2020-07-03T15:16:58Z
dc.date.issued2019-07-25
dc.identifier.urihttp://eteze.kg.ac.rs/application/showtheses?thesesId=7100
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/11998
dc.identifier.urihttps://fedorakg.kg.ac.rs/fedora/get/o:1200/bdef:Content/download
dc.description.abstractCilj ove studije je bio da se odredi uticaj ekspresije receptora za estrogene i progesteron na angiogenezu, proliferaciju i apoptozu ćelija mioma kod žena u pre i postmenopauzi. Ovo je bila studija preseka, kliničko-eksperimentalna, retrospektivna, neintervencijska studija u polju istraživanja fundamentalnih mehanizama patogeneze bolesti, korišćenjem patohistološkog materijala iz postojeće arhive. Istraživanjem je obuhvaćeno 76 pacijentkinja sa dijagnostikovanim lejomioima uterusa, operativno lečenim na Klinici za ginekologiju i akušerstvo, Kliničkog centra Kragujevac, Srbija. Prema menstrualnom statusu, formirane su dve eksperimentalne podgrupe. Prva grupa bile su žene u premenopauzi (PreM) (n = 35; 46,2 ± 5,02 godina), a druga grupa bile su žene u postmenopauzi (PostM) (n = 41; 60,25 ± 5,41 godina). Korišćen je metod standardnog H&E bojenja za isečke mioma, miometrijuma i endometrijuma, kao i imunohistohemijski metod za određivanje ekspresije ERα, ERβ, PRa, VEGF, CD105, Ki- 68 i Caspase -3. Ekspresija receptora za progesteron je bila više izražena u miomima i miometrijumu kod premenopauznih žena u poređenju sa miomima i miometrijumom kod žena u postmenopauzi. Ekspresija Caspase-3 je statistički značajno veća u grupi PostM žena u poređenju sa PreM grupom. Ekspresija ERα i ERβ nije bila značajno različita imeđu grupa ni u uzorcima mioma ni miometrijuma. Prema našim podacima, ekspresija receptora za progesteron je imala veći uticaj na apoptozu i proliferaciju ćelija nego ekspresija receptora za estrogene. Povećana ekspresija receptora za progesteron kod žena u PreM u miomu i miometrijumu, verovatno je dovela do smanjenja ekspresije apoptotskog markera kod PreM žena.sr
dc.description.abstractThe aim of this study was to determine the effects of the estrogens and progesterone receptors status on angiogenesis, proliferation and apoptosis of myoma cells in premenopausal and postmenopausal women. This was a cross section, clinical-experimental, retrospective, noninterventional study in the field of the study of fundamental pathogenesis mechanisms of disease using pathohistological materials from the existing archive. The research included 76 patients diagnosed with uterine leiomyomas, operatively treated in the Clinic for Gynecology and Obstetrics, Clinical Centre Kragujevac, Serbia. According to the menstrual status, two experimental subgroups were formed. The first group was premenopausal women (PreM) (n=35; 46.2±5.02 year old), and the second group was postmenopausal women (PostM) (n=41; 60.25±5.41 year old). H&E staining for myoma and myometrium was conducted as well as immunohistochemistry for ERα, ERβ, PRа, VEGF, CD105, Ki678 and Cas-3. Progesterone receptor was over expressed in myoma and myometrium of premenopausal compared to myoma and myometrium of postmenopausal women. Expression of Caspase 3 was statistically significant increased in PostM women compared to PreM group. ERα and ERβ were not changed among groups neither in myoma nor in myometrium samples. According to our data, PRа had higher influence on apoptosis and cell growth than estrogen receptors. Since PRа was increased in PreM in both myoma and myometrium, probably this expression led further to lower expression of apoptotic marker in PreM women.en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Крагујевцу, Факултет медицинских наука
dc.rightsopenAccessen
dc.sourceУниверзитет у Крагујевцуsr
dc.subjectlejomiomi materice, steroidni receptori, proliferacija, apoptoza, angiogenezasr
dc.subjectUterine leiomyoma, steroid receptors, proliferation, apoptosis, angiogenesisen
dc.titleUticaj ekspresije estrogenih i progesteronskih receptora na angiogenezu, apoptozu i proliferaciju ćelija mioma kod žena u pre i postmenopauzisr
dc.title.alternativeThe influence of the expression estrogens and progesterone receptors on angiogenesis, proliferation and apoptosis of myoma cells in premenopausal and postmenopausal womenen
dc.typedoctoralThesis
dc.rights.licenseBY-NC-ND
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/49120/Dusko_Kljakic_Medicinski.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/49119/Disertacija.pdf


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