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Cytokine genes polymorphisms of TNF-[alpha] , IFN-[gama] and IL-12 as potential predictors in the onset of cervical disease associated with HR-HPV infections

dc.contributor.advisorĆupić, Maja
dc.contributor.otherPravica, Vera
dc.contributor.otherPerišić, Živko
dc.contributor.otherLazarević, Ivana
dc.contributor.otherKnežević, Aleksandra
dc.creatorTasić, Dijana M.
dc.date.accessioned2020-02-14T14:30:38Z
dc.date.available2020-02-14T14:30:38Z
dc.date.available2020-07-03T08:52:24Z
dc.date.issued2019-09-30
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/11844
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=7204
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:20902/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51750415
dc.description.abstractKarcinom grlića materice predstavlja četvrti po učestalosti maligni tumor kod žena. Infekcija visoko onkogenim tipovima HPV igra centralnu ulogu u razvoju cervikalne intraepitelijalne neoplazije i cervikalnog karcinoma. Pored HR-HPV, brojni kofaktori igraju značajnu ulogu u malignoj transformaciji ćelija. Najznačajniji među njima su faktori domaćina (genetski faktori i imunski status), faktori spoljašnje sredine i bihejvioralni faktori (multiparitet, upotreba oralnih kontraceptiva, pušenje, infekcija drugim seksualno prenosivim patogenim uzročnicima, ranije stupanje u seksualne odnose, promiskuitet, loši socioekonomski uslovi i nutritivni faktori). Ciljevi istraživanja: Cilj ove studije bio je da se kod ispitanica, koje su sa prethodno definisanim kolposkopskim i citološkim nalazom bile grupisane u četiri kategorije: PAP II, ASCUS, LSIL, HSIL, utvrdi prisustvoHR-HPV. Takođe je kod svih HR-HPV pozitivnih žena trebalo ispitati distribuciju genotipova i alela proinflamatornih citokina TNF-α,IFN-γ i IL-12 i njihov uticaj na nastanak cervikalne bolesti udružene sa HR-HPV i utvrditi koji genotipovi imaju protektivni, odnosno favorizujući, značaj za nastanak karcinoma grlića materice. Takođe, cilj istraživanja bio je da se kod svih HR-HPV pozitivnih pacijentkinja na osnovu upitnika utvrde kofaktori rizika za nastanak cervikalne bolesti i ispita povezanost između kofaktora i konstitucionalnih genskih polimorfizama proinflamatornih citokina, kao i njihov zajednički uticaj na nastanak cervikalnog karcinoma. Materijal i metodologija: Istraživanje dizajnirano kao studija preseka sprovedeno je tokom četvorogodišnjeg perioda, od 2014. do 2018. godine i obuhvatilo je 541 pacijentkinju. Svim pacijentkinjama je tokom ginekološkog pregleda utvrđen kolposkopski i citološki status na grliću materice. Na osnovu citološkog nalaza sve pacijentkinje podeljene su u četiri grupe: PAP II, ASCUS, LSIL, HSIL...sr
dc.description.abstractCervical cancer is the fourth most common malignant tumor found in women worldwide. Infection with high risk onconegic HPV types plays a central role in the development of cervical precursor lesion and cervical cancer. In addition to HR-HPV infection, numerous cofactors are important for malignant cell transformation. The most important among theme are: host factors (genetic and immune factors), environmental and behavioral factors (multiparity, use of oral contraceptives, smoking, sexually transmitted infections, early sexual intercourse, promiscuity, poor socio-economic conditions and nutritional factors). Aims: The aim of this study was to determinate the presence of HR-HPV infection in women that were previously divided into four groups (PAP II, ASCUS, LSIL, HSIL) based on cytological and colposcopic findings. In all HR-HPV positive women, it was also necessary to investigate the distribution of genotypes and allele of proinflammatory cytokines TNF-α, IFN-γ and IL-12 and their effect on the development of a cervical illness associated with HR-HPV and also to determine which genotypes possese a protective or favoring significance for the development of cervical cancer. Further aims were to establish risk factors for the occurrence of cervical disease in all HR-HPV positive patients and to examine the association between this cofactors and constitutional gene polymorphisms of proinflammatory cytokines, as well as their common influence on the development of cervical carcinoma. Material and methodology: This four year cross sectional study was conduced from 2014 to 2018 and included 541 patients. Colposcopic and cytological status was determinated for each patients during the gynecological examination. Based on the cytological findings, all patients were classifeid into four groups: PAP II, ASCUS, LSIL, HSIL. To determinate the presence of HPV infection and single nucleotide polymorphism of TNF-α, IFN-γ and IL-12, cervical swab were taken from visible lesions on the ectocervix using cervical cytobras. Further procedure involved the isolation of the total DNA using a commercial DNA extraction kit...en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Медицински факултетsr
dc.rightsopenAccessen
dc.sourceУниверзитет у Београдуsr
dc.subjectHR-HPVsr
dc.subjectpolymorphismen
dc.subjectpolimorfizmisr
dc.subjectTNF-sr
dc.subjectIFN-sr
dc.subjectIL-12sr
dc.subjectCINsr
dc.subjectcervikalni karcinomsr
dc.subjectTNF-αen
dc.subjectIFN-γen
dc.subjectIL-12en
dc.subjectHR-HPV infectionen
dc.subjectCINen
dc.subjectcervical canceren
dc.titlePovezanost polimorfizama gena za TNF-a, IFN-y i IL-12 sa nastankom cervikalne intraepitelijalne neoplazije kod žena sa infekcijom humanim papiloma virusomsr
dc.title.alternativeCytokine genes polymorphisms of TNF-[alpha] , IFN-[gama] and IL-12 as potential predictors in the onset of cervical disease associated with HR-HPV infectionsen
dc.typedoctoralThesis
dc.rights.licenseBY-NC-ND
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/10583/IzvestajKomisije21870.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/10582/Disertacija.pdf


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