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Morphological, phytochemical and molecular studies of Carpathian-Balkan complex Centaurea atropurpurea (Asteraceae) - phylogenetic and taxonomic implications

dc.contributor.advisorJanaćković, Peđa
dc.contributor.otherMarin, Petar
dc.contributor.otherLakušić, Dmitar
dc.contributor.otherZlatković, Bojan
dc.contributor.otherRajčević, Nemanja
dc.creatorNovaković, Jelica J.
dc.date.accessioned2020-02-07T10:29:23Z
dc.date.available2020-02-07T10:29:23Z
dc.date.available2020-07-03T08:08:13Z
dc.date.issued2019-10-19
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/11825
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=7179
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:20872/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025234354
dc.description.abstractPredstavnici kompleksa Centaurea atropurpurea (Acrocentron, Asteraceae) naseljavaju Balkan i južne Karpate. Varijabilnost taksona ovog kompleksa navodi na potrebu detaljnog istraživanja, kako bi se razjasnio njihov taksonomski status i bolje razumela filogenija grupe. Morfološke, fitohemijske, citološke i molekularne analize obuhvatile su 23 populacije vrste Centaurea calocephala sa celog areala, kao i 16 populacija ostalih taksona iz kompleksa Centaurea atropurpurea. Na osnovu horoloških podataka urađene su mape distribucije pojedinačnih taksona i istraživanog kompleksa. Opisana je nova vrsta za nauku – Centaurea zlatiborensis, endemična vrsta za Srbiju. Urađena je lektotipifikacija C. gjurasinii i C. crnogorica. Rezultati morfoloških analiza pokazali su veću varijabilnost karaktera stabljike i listova nego karaktera cvasti. Klaster analiza i CDA populacija C. calocephala pokazale su izdvajanje populacija: 1 (Lika) i 17 (Cluj-Napoca) na osnovu karaktera cvasti, dok su se populacije: 17 (Cluj-Napoca) i 7 (Đerdap) izdvojile na osnovu karaktera stabljike i listova. Fitohemijska ispitivanja isparljivih komponenti taksona kompleksa Centaurea atropurpurea pokazala su prisustvo dominantnih komponenti: germakrena D, E-kariofilena i kariofilen oksida. Na osnovu fitohemijskih markera u okviru taksona C. calocephala izdvojile su populacije 1 (Lika) i 20 (Cheile Turzii), dok su se taksoni C. zlatiborensis i C. crnogorica izdvojili od ostalih takona kompleksa. ETS analiza pokazala je monofiletsko poreklo Balkanske klade sek. Acrocentron. Uočena je introgresija i retikulacija koje su posledice homoploidne hibridizacije između taksona tokom ledenog doba u refungijumima na Balkanu. Na osnou ETS i AGT1 izdvojile su se grupe Calocephala A i B, kao i Grbavacensis grupa. Citološke analize pokazale su da svi istraživani taksoni iz kompleksa imaju diploidan broj hromozoma: 2n=20 i 2n=22.sr
dc.description.abstractRepresentatives of Centaurea atropurpurea complex (Acrocentron, Asteraceae) are distributed on the Balkans and the southern Carpathians. The variability of the taxa of this complex indicates the need for detailed research, in order to clarify their taxonomic status and phylogeny. Morphological, phytochemical, cytological and molecular analyses included twenty-three populations of Centaurea calocephala and sixteen populations of other taxa from Centaurea atropurpurea complex. Based on the horological data, maps of the distribution for each taxon and the investigated complex were made. A new species for science has been described ‒ Centaurea zlatiborensis, an endemic species for Serbia. C. gjurasinii and C. crnogorica are lectotypified. The results of morphological analyses showed a greater variability of the stem and the leaves characters in comparison to the capitula characters. In cluster analysis and CDA population of C. calocephala separated from populations: 1 (Lika) and 17 (Cluj-Napoca) based on the characters of capitula, while the populations: 17 (Cluj-Napoca) and 7 (Djerdap) were separated based on the characters of the stem and leaves. Phytochemical analysis of the volatile compounds of the Centaurea atropurpurea complex showed the presence of the dominant components: germacrene D, E-caryophyllene and caryophyllene oxide. On the basis of the phytochemical markers, the populations of 1 (Lika) and 20 (Cheile Turzii) were separated within the C. calocephala, while the C. zlatiborensis and C. crnogorica separated from other taxa of the complex. ETS analysis showed the monophyletic origin of the Balkan clade of the sect. Acrocentron. Introgression and reticulation were observed, which are the consequences of homoploid hybridization between taxa during the ice age in Balkan refugia. ETS and AGT1 showed forming the groups Calocephala A and B, as well as the Grbavacensis group. Cytological analyses have shown that all investigated taxa from the complex have a diploid chromosome number: 2n = 20 and 2n = 22.en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Биолошки факултетsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173029/RS//
dc.rightsopenAccessen
dc.sourceУниверзитет у Београдуsr
dc.subjectAsteraceaesr
dc.subjectAsteraceaeen
dc.subjectleavesen
dc.subjectand stemsen
dc.subjectlistova i stabljikesr
dc.subjectisparljive komponentesr
dc.subjectETS i AGT1sr
dc.subjectbroj hromozomasr
dc.subjectdistribucijasr
dc.subjecttaksonomijasr
dc.subjectfilogenijasr
dc.subjectvolatile componentsen
dc.subjectETS and AGT1en
dc.subjectchromosome numberen
dc.subjectdistributionen
dc.subjecttaxonomyen
dc.subjectphylogenyen
dc.subjectkompleks Centaurea atropurpureasr
dc.subjectmorfologija cvastisr
dc.subjectcomplex Centaurea atropurpureaen
dc.subjectmorphology of capitulaen
dc.titleMorfološka, fitohemijska i molekularna istraživanja karpatsko - balkanskog kompleksa Centaurea atropurpurea (Asteraceae) - filogenetski i taksonomski aspektsr
dc.title.alternativeMorphological, phytochemical and molecular studies of Carpathian-Balkan complex Centaurea atropurpurea (Asteraceae) - phylogenetic and taxonomic implicationsen
dc.typedoctoralThesis
dc.rights.licenseBY-NC-ND
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/1827/Disertacija.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/1828/IzvestajKomisije21835.pdf


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