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Analysis of tumor necrosis factor [alpha], interleukin 6 and interleukin 1[beta] gene polymorphisms frequency in preterm infants as risk factors for development of sepsis

dc.contributor.advisorDamjanović, Tatjana
dc.contributor.otherVujić, Vesna
dc.contributor.otherPravica, Vera
dc.contributor.otherJešić, Miloš
dc.contributor.otherPavlekić, Snežana
dc.creatorVarljen, Tatjana
dc.date.accessioned2019-12-24T16:07:29Z
dc.date.available2019-12-24T16:07:29Z
dc.date.available2020-07-03T08:46:05Z
dc.date.issued2019-09-25
dc.identifier.urihttps://nardus.mpn.gov.rs/handle/123456789/11634
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=7000
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:20534/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51747855
dc.description.abstractNeonatalna sepsa predstavlja vodeći uzrok morbiditeta i mortaliteta kod prevremeno rođene dece. Genetički faktori mogu uticati na nastanak, tok i ishod rane neonatalne sepse. Cilj našeg istraživanja bio je da ispitamo povezanost TNFα -308 G/A, IL6 -174 G/C i IL1β -511 G/A polimorfizama sa nastankom i ishodom rane neonatalne sepse kod prematurusa. Istraživanje je obuhvatilo 471 prevremeno rođeno dete, 282 sa sepsom (151 hemokulturom potvrđenom i 131 kliničkom sepsom) i 189 bez sepse koji su činili kontrolnu grupu. Učestalost alela A i AA genotipa TNFα -308 G/A polimorfizma je statistički značajno veća kod prematurusa sa sepsom u odnosu na prematuruse kontrolne grupe. GA+AA genotipovi polimorfizma TNFα -308 G/A su statistički značajno povezani sa nastankom hemokulturom potvrđene sepse i kliničke sepse. Učestalost AA genotipa IL1β -511 G/A polimorfizma statistički je značajno veća u grupi prematurusa sa hemokulturom potvrđenom sepsom i kliničkom sepsom u odnosu na kontrolnu grupu. Takođe, AA genotip IL1β -511 G/A polimorfizma udružen je sa letalnim ishodom od sepse kod prematurusa. Logistička regresiona analiza sa gestacijskom starošću, porođajnom telesnom masom i Apgar skorom u ’5 kao kovarijatama potvrdila je statistički značajnu povezanost geneotipova GA+AA TNFα -308 G/A i AA IL1β -511 G/A polimorfizma sa nastankom sepse. U našoj studiji nema statistički značajne razlike u učestalosti alela i genotipova IL6 -174 G/C polimorfizma između grupa prematurusa sa hemokulturom potvrđenom, kliničkom sepsom i kontrolne grupe prematurusa. Rezultati naše studije pokazuju da su alel A i AA genotip TNFα -308 G/A i AA genotip IL1β -511 G/A polimorfizma faktori rizika za razvoj rane neonatalne sepse. Genotip AA IL1β -511 G/A polimorfizma je faktor rizika za smrtni ishod od rane neonatalne sepse prematurusa.sr
dc.description.abstractNeonatal sepsis is the major cause of morbidity and mortality among preterm infants. Genetic predisposition may have an impact on sepsis susceptibility and outcome. The aim of our study was to explore the association of TNFα -308 G/A, IL-6 -174 G/C and IL-1β -511 G/A gene polymorphisms with susceptibility and outcome of early onset sepsis (EOS) in preterm infants. Study included 471 preterm infants, 282 with EOS (culture proven sepsis 151 and clinical sepsis 131) and 189 as a control group. We observed significantly higher frequency of A allele and AA genotype of TNFα -308 G/A polymorphism in blood culture proven EOS or clinical EOS compared to the control group. Statistically significant difference was observed in TNFα -308 G/A genotypes distribution between sepsis and control groups by dominant model (GG/GA+AA). The frequencies of A allele and AA genotype of IL-1β -511G/A polymorphism are significantly higher in clinical EOS and blood culture proven EOS compared to the control group. A significant difference was observed between clinical EOS and blood culture proven EOS and control group by recessive model (GG+GA/AA). Our study has shown that AA genotype of IL-1β -511 G/A polymorphism was a significant predictor of lethal outcome. Logistic regression with BMW, gestational age, and Apgar score as covariates confirmed significant association between TNFα -308 G/A and IL-1β -511 G/A genotypes and development of sepsis. Genotype and allele frequencies of IL6 -174G/C polymorphism were not significant different between culture proven EOS group, clinical EOS group and the control group did not show significant differences. According to our results TNFα -308 A allele and AA genotype and IL-1β -511 AA genotype may be risk factors for the development of EOS while IL-1β -511 AA genotype may be predictor of EOS lethal outcome.en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Медицински факултетsr
dc.rightsopenAccessen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceУниверзитет у Београдуsr
dc.subjectTNFα -308 G/A polimorfizamsr
dc.subjectTNFα -308 G/A polymorphismen
dc.subjectIL-6 -174 G/C polimorfizamsr
dc.subjectIL-1β -511 G/A poimorfizamsr
dc.subjectrana neonatalna sepsasr
dc.subjectletalni ishodsr
dc.subjectIL6 -174 G/C polymorphismen
dc.subjectIL1β -511 G/A polymorphismen
dc.subjectearly onset sepsisen
dc.subjectlethal outcomeen
dc.titleIspitivanje učestalosti polimorfizama gena za faktor nekroze tumora [alfa], interleukin 6 i interleukin 1[beta] kod prevremeno rođene dece kao faktor rizika za nastanak sepsesr
dc.title.alternativeAnalysis of tumor necrosis factor [alpha], interleukin 6 and interleukin 1[beta] gene polymorphisms frequency in preterm infants as risk factors for development of sepsisen
dc.typedoctoralThesisen
dc.rights.licenseBY
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/8224/Disertacija.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/8225/IzvestajKomisije21220.pdf
dc.identifier.fulltexthttps://nardus.mpn.gov.rs/bitstream/id/8224/Disertacija.pdf
dc.identifier.fulltexthttps://nardus.mpn.gov.rs/bitstream/id/8225/IzvestajKomisije21220.pdf
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_nardus_11634


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