Ispitivanje antioksidativne i antitumorske aktivnosti ekstrakata tri odabrane vrste lišajeva Cladonia subulata, Pleurosticta acetabulum i Physcia semipinnata
Examination of the antioxidant and antitumor activity of the extracts of three selected lichen species Cladonia subulata, Pleurosticta acetabulum and Physcia semipinnata and Physcia semipinnata
Author
Tomović, JovicaMentor
Manojlović, NedeljkoCommittee members
Milovanović, DraganVasiljević, Perica
Milovanović, Marija
Metadata
Show full item recordAbstract
Cilj ove doktorske disertacije je ispitivanje hemijskog sastava, antioksidativne i
antitumorske aktivnosti ekstrakata tri vrste lišajeva: Cladonia subulata, Pleurosticta
acetabulum i Physcia semipinnata. Ispitivanje hemijskog sastava acetonskih i
metanolskih ekstrakata obuhvatao je HPLC-UV analizu ekstrakata kao i određivanje
ukupnog sadržaja fenola i flavonoida u ekstraktima. Ispitivani esktrakti su pokazali
visok sadržaj ukupnih fenola (21,31±1,19 do 73,45±0,82 mg GA/g) i flavonoida
(8,48±0,57 do 19,27±0,37 mg RU/g). Ispitivanje ekstrakata lišajeva HPLC-UV analizom
potvrđeno je prisustvo dve grupe sekundarnih metabolita depsidona i depsida:
hipoprotocetrarinska kiselina, fumaroprotocetrarinska kiselina, salazinska
kiselina, norstihninska kiselina, protocetrarinska kiselina, evernijska kiselina,
atranorin, lekanorna kiselina i obtusinska kiselina. Ispitivanje biološke aktivnosti
ekstrakata obuhvatalo je evaluaciju antioksidativne i antitumorske aktivnosti.
Antioksidativna ...aktivnost je utvrđena praćenjem ukupnog antioksidativnog kapaciteta,
sposobnosti neutralizacije DPPH radikala, sposobnosti neutralisanja hidroksil
radikala, redukcinog potencijala i inhibicije lipidne peroksidacije. Rezultati
ispitivanja in vitro antioksidativne aktivnosti pokazuju da ekstrakti ispitivanih vrsta
lišajeva poseduju značajan antioksidativni potencijal. Antitumorska aktivnost
ekstrakata lišajeva procenjena je korišćenjem MTT testa ispitivanjem vijabilnosti i
proliferacije na ćelijama Hela S3 (adenokarcinom cerviksa) i LS174 (ćelije karcinoma
debelog creva). Ispitivani ekstrakti pokazali su antitumorsku aktivnost u različitim
koncetracijama (IC50=13,55 do >200 μg/ml), u zavisnosti od ekstrakta i ispitivane
ćelijske linije. Na osnovu dobijenih rezultata, može se prepostaviti da vrste lišajeva
Cladonia subulata, Pleurosticta acetabulum i Physcia semipinnata u budućnosti mogu naći
svoju potencijalnu primenu u medicini, farmaceutskoj, prehrambenoj i kozmetičkoj
industriji, kao i u razvoju novih fitopreparata.
The aim of this doctoral thesis is to investigate the chemical composition, antioxidant and
antitumor activity of the extracts of three lichen species: Cladonia subulata, Pleurosticta
acetabulum and Physcia semipinnata. Examination of the chemical composition of acetone and
methanol extracts included the HPLC-UV analysis of the extracts as well as the determination of
the total content of phenol and flavonoids in the extracts. The examined extracts showed a high
content of total phenols (21.31 ± 1.19 to 73.45 ± 0.82 mg GA/g) and flavonoids (8.48 ± 0.57 to
19.27 ± 0.37 mg RU/g). Examination of extracts of lichens by HPLC-UV analysis confirmed the
presence of two groups of secondary metabolites of depsidone and depside: hypoprotocetraric
acid, fumaroprotocetraric acid, salazinic acid, norstictic acid, protocetraric acid, evernic acid,
atranorine, lecanoric acid and obtusic acid. The study of the biological activity of extracts
involved the evaluation of antioxidant and antitumo...r activity. The antioxidant activity was
determined by monitoring the total antioxidant capacity, the ability to neutralize DPPH radicals,
the ability to neutralize hydroxyl radicals, reducing potential, and inhibiting lipid peroxidation.
The results of an in vitro antioxidative activity test show that the extracts of the tested lichen
species have a significant antioxidant potential. Antitumor activity of lichen extracts was
assessed using the MTT test by testing viability and proliferation of Hela S3 cells (cervix
adenocarcinoma) and LS174 (colon cancer cells). The investigated extracts showed antitumor
activity in different concentrations (IC50 = 13.55 to> 200 μg / ml), depending on the extract and
the cell line examined. On the basis of the obtained results, it can be assumed that the species of
lichens of Cladonia subulata, Pleurosticta acetabulum and Physcia semipinnata can find their
potential application in the medical, pharmaceutical, food and cosmetic industries, as well as in
the development of new phytopharmaceuticals, in the future.
Faculty:
Универзитет у Крагујевцу, Факултет медицинских наукаDate:
05-02-2019Projects:
- Dynamics of nonlinear physicochemical and biochemical systems with modeling and predicting of their behavior under nonequilibrium conditions (RS-172015)
- Natural products of plants and lichens: isolation, identification, biological activity and application (RS-172047)
- Characterization and application of fungal metabolites and assessment of new biofungicides potential (RS-173032)