Istraživanje influence kopitara sa različitim epizootiološkim i kliničkim nalazima infekcije
Research of equine influenza with different epizootic and clinical findings of infection
Abstract
Influenca kopitara je ozbiljno, akutno, visoko kontagiozno, respiratorno oboljenje kopitara sa karakterističnom kliničkom slikom u tipu respiratornog simptoma. Ovo oboljenje izazivaju dva suptipa influenca A virusa (EIV – ekvini influenca virus) – H7N7 (ranije poznat kao tip 1) i H3N8 (ranije poznat kao tip 2) iz familije Orthomyxoviridae. Cilj istraživanja je bio da se uporede različite dijagnostičke metode za dijagnostiku influenca A virusa kod kopitara sa različitim vakcinalnim statusom i kliničkim nalazom. U eksperimentu je učestvovalo 61 grlo. Sprovedena je epizootiološka anketa, grla su klinički pregledana, analizirana je kompletna krvna slika, urađen HI, Direktigen i RT-PCR test. Rezultati su pokazali prisustvo oba suptipa virusa. Registrovane su seropozitivne jedinke kod kojih je dokazano prisustvo antigena virusa influence, ali bez dokazanog prisustva genoma virusa influence.
Equine influenza is a serious, acute, highly contagious, respiratory disease, equine with characteristic clinical picture in the type of respiratory syndrome. This disease is caused by two subtypes of influenza A virus (EIV - equine influenza virus) - H7N7 (formerly known as type 1) and H3N8 (formerly known as type 2) from the family Orthomyxoviridae. The aim of the research was to compare different diagnostic methods for the diagnosis of influenza A virus in equine vaccine with different status and clinical findings. The experiment involved 61 throat. Conducted epidemiological surveys, clinical throat examined, analyzed the complete blood count, done HI, Directigen and RT-PCR test. The results showed the presence of both subtypes of the virus. Seropositive individuals are registrated in which the presence of an influenza virus antigen is confirmed, but without evidence of the presence of the influenza virus genome.