Приказ основних података о дисертацији

dc.contributor.advisorRadinović, Miodrag
dc.contributor.advisorPetrović, Kosta
dc.contributor.otherBoboš, Stanko
dc.contributor.otherSavić, Sara
dc.contributor.otherPajić, Marija
dc.creatorGalfi, Annamaria
dc.date.accessioned2019-01-18T10:40:10Z
dc.date.available2019-01-18T10:40:10Z
dc.date.available2020-07-03T13:33:02Z
dc.date.issued2016-07-27
dc.identifier.urihttps://nardus.mpn.gov.rs/handle/123456789/10646
dc.identifier.urihttps://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija146313690647615.pdf?controlNumber=(BISIS)100966&fileName=146313690647615.pdf&id=5617&source=NaRDuS&language=srsr
dc.identifier.urihttps://www.cris.uns.ac.rs/record.jsf?recordId=100966&source=NaRDuS&language=srsr
dc.identifier.urihttps://www.cris.uns.ac.rs/DownloadFileServlet/IzvestajKomisije146313692757124.pdf?controlNumber=(BISIS)100966&fileName=146313692757124.pdf&id=5618&source=NaRDuS&language=srsr
dc.description.abstractKontrola zdravlja vimena krava je bitan element u procesu proizvodnje zdravstveno bezbednog mleka, te se na farmama visokomlečnih krava, kroz program kontrole mastitisa, redovno sprovode mere otkrivanja i prevencije bolesti vimena. Klinički pregled vimena predstavlja osnovni metod koji pruţa korisne informacije o zdravstvenom statusu vimena krava, ali nailazi na poteškoće u otkrivanju patoloških promena unutar parenhima i papile vimena. U cilju otkrivanja promena u parenhimu vimena moţe se primeniti ultrazvučni pregled koji omogućava vizualizaciju strukturnih promena vimena nastalih kao posledica upalnih procesa i tako olakšava dijagnostiku oboljenja. Tokom poslednjih godina, javio se problem povećanja rezistencije bakterija na antimikrobne lekove, što oteţava lečenje bolesti, ali i ugroţava zdravlje ţivotinja i ljudi. Najčešći uzroci toga su nepravilna upotreba i eventualna zloupotreba antimikrobnih lekova. Mnoga istraţivanja vršena su u in vitro i in vivo uslovima na primeni laktoferina samog ili u kombinaciji sa antibioticima u terapiji i prevenciji mastitisa krava. Laktoferin, gvoţĎe vezujući antimikrobni glikoprotein koji se nalazi u mleku i drugim sekretima, predstavlja bitan deo sistema odbrane mlečne ţlezde. Cilj istraţivanja u okviru ove disertacije je procena dijagnostičke mogućnosti ultrazvučnog pregleda u detekciji subkliničkog mastitisa i poremećene sekrecije vimena, kao i razmatranje opravdanosti primene laktoferina u prevenciji i lečenju mastitisa. Kliničkim pregledom izvršena je procena opšteg zdravstvenog stanja krava, kao i ispitivanje mlečne ţlezde adspekcijom i palpacijom. Za otkrivanje poremećene sekrecije vimena i subkliničkih mastitisa korišćeni su brzi testovi, Kalifornija mastitis test i Draminski test, kao i ultrazvučni pregled mlečne ţlezde krava. OdreĎivanje broja somatskih ćelija u uzorcima mleka uraĎeno je metodom protočne citometrije. Za identifikaciju uzročnika mastitisa korišćene su klasične mikrobiološke metode. Krave sa pozitivnim bakteriološkim nalazom podeljene su u dve ogledne grupe. Krave ogledne grupe I su tretirane intramamarnom aplikacijom antibiotika, dok je kravama ogledne grupe II aplikovana kombinacija antibiotika i laktoferina. OdreĎivanje koncentracije imunoglobulina G u mlečnom serumu krava vršeno je metodom radioimunodifuzije, a odreĎivanje koncentracije laktoferina u mleku krava ELISA testom. U istraţivanjima u okviru ove disertacije, najčešće izolovani major mastitis patogeni bile su bakterije Staphylococcus aureus i Streptococcus agalactiae, a najčešće izolovani minor mastitis patogeni Corynebacterium spp. i koagulaza negativne stafilokoke. Ultrasonografija mlečne ţlezde krava pokazala se kao efikasna metoda u dijagnostici poremećaja sekrecije vimena. Veće vrednosti koncentracije imunoglobulina G u mlečnom serumu krava uočene su tokom perioda predzasušenja i zasušenja, u odnosu na period rane laktacije. Najveći uticaj na porast koncentracije laktoferina u mleku krava su imali bakterijski uzročnici mastitisa. Efikasnost antibiotske terapije primenjene tokom perioda zasušenja kod krava ogledne grupe I iznosila je 52,7%, dok je efikasnost primenjene terapije sa goveĎim laktoferinom i antibiotikom kod krava ogledne grupe II iznosila 60%. Aplikacija laktoferina tokom perioda zasušenja doprinela je efikasnosti terapije intramamarnih infekcija, ali nije imala uticaj na koncentraciju laktoferina u mleku tokom perioda rane laktacije. Dalja istraţivanja su neophodna kako bi se u potpunosti potvrdila efikasnost intramamarne terapije sa goveĎim laktoferinom i antibiotikom u cilju eliminacije uzročnika mastitisa i da se precizno odredi terapijska koncentracija laktoferina.sr
dc.description.abstractControl of udder health is an essential element in the process of safe milk production, thus, through the mastitis control program, dairy farms regularly conduct measures of detection and prevention of udder diseases. Clinical examination of the udder is a basic method that provides useful information about the health status of cow udders, but this method is limited in its ability to detect pathological changes in the udder parenchyma and teat. In order to detect changes in the parenchyma of the udder, an ultrasound can be applied which allows visualization of udder structural changes incurred as a result of inflammatory processes, thus facilitating diagnosis of diseases. Over the last few years, the problem of increasing resistance to antimicrobial drugs has appeared, making it difficult to treat disease and also threaten the health of animals and humans. The incorrect and widespread use of antimicrobial drugs are the most common cause of this. Many studies were conducted in vitro and in vivo conditions on the use of lactoferrin alone or in combination with antibiotics in the treatment and prevention of mastitis. Lactoferrin, an iron-binding antimicrobial glycoprotein which is found in milk and other secretions, represents an important part of the mammary gland defense system. The aim of the research within this thesis was to evaluate the diagnostic possibilities of udder ultrasonography in the detection of subclinical mastitis and udder secretion disorders, as well as considering the feasibility of lactoferrin application in the prevention and treatment of mastitis. The general condition of the animals was evaluated by clinical examination, as well as udder examination by adspection and palpation. The California mastitis test, Draminski test and ultrasound examination of the cow's mammary glands were used for detection of udder secretion disorders and subclinical mastitis. Somatic cell count in milk samples was determined by flow cytometry method. Classical microbiological methods were used for the isolation and identification of mastitis causative agents. Cows with positive bacteriological findings were divided into two experimental groups. Cows in experimental group I were treated with intramammary applications of antibiotics, while the cows in experimental group II were treated with a combination of antibiotics and lactoferrin. Immunoglobulin G concentration in bovine milk serum was determined by the immunodiffusion method, while lactoferrin concentration in bovine milk was determined using the ELISA test. In this study, the most common isolated major mastitis pathogens were Staphylococcus aureus and Streptococcus agalactiae, while Corynebacterium spp. and coagulase-negatice staphilococci were the most commonly detected minor mastitis pathogens. Ultrasonography of the bovine mammary gland proved to be an effective method in the diagnosis of udder secretion disorders. Higher immunoglobulin G concentrations were observed in milk serum from cows during pre-dry and dry period, relative to early lactation period. The biggest influence on the increase in the concentration of lactoferrin in the bovine milk had mastitis pathogens. The efficacy of antibiotic therapy during the dry period in cows of experimental group I was 52.7%, while the efficacy of the applied therapy with lactoferrina and antibiotics in cows of experimental group II was 60%. Application of lactoferrin during the dry period contributed to the effectiveness of the treatment of intramammary infections, but had no influence on lactoferrin concentration in the milk during the early lactation period. Further studies are necessary to in order to fully confirm the efficacy of intramammary therapy with bovine lactoferrin and antibiotic to eliminate the mastitis pathogens and to determine the therapeutic concentration of lactoferrin.en
dc.languagesr (latin script)
dc.publisherУниверзитет у Новом Саду, Пољопривредни факултетsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31034/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/23032/RS//
dc.rightsopenAccessen
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceУниверзитет у Новом Садуsr
dc.subjectvimesr
dc.subjectudderen
dc.subjectcowen
dc.subjectmilken
dc.subjectlactoferrinen
dc.subjectimmunoglobulin Gen
dc.subjectultrasonographyen
dc.subjectudder pathogensen
dc.subjectkravasr
dc.subjectmlekosr
dc.subjectlaktoferinsr
dc.subjectimunoglobulin Gsr
dc.subjectultrasonografijasr
dc.subjectpatogeni vimenasr
dc.titleKlinički i ultrazvučni pregled vimena krava nakon primenelaktoferina u periodu involucijesr
dc.typedoctoralThesisen
dc.rights.licenseBY-NC-ND
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/36037/IzvestajKomisije.pdf
dc.identifier.fulltexthttps://nardus.mpn.gov.rs/bitstream/id/36036/Disertacija.pdf
dc.identifier.fulltexthttps://nardus.mpn.gov.rs/bitstream/id/36037/IzvestajKomisije.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/36036/Disertacija.pdf
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_nardus_10646


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