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The impact of systemic administration of lipopolysaccharide on structural, cellular and molecular characteristics of the mouse spleen.

dc.contributor.advisorMilićević, Novica
dc.contributor.otherBumbaširević, Vladimir
dc.contributor.otherPopadić, Dušan
dc.contributor.otherČolić, Miodrag
dc.creatorLalić, Ivana M.
dc.date.accessioned2019-01-11T13:29:39Z
dc.date.available2019-01-11T13:29:39Z
dc.date.available2020-07-03T08:49:53Z
dc.date.issued2018-09-20
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/10570
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=6427
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:19142/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50762255
dc.description.abstractLipopolisaharid (LPS) je glavna komponenta spoljašnje membrane Gram negativnih bakterija. Prepoznavanje LPS-a inicira intracelularne signalne puteve koji dovode do transkripcije gena i oslobaĊanja proinflamatornih medijatora. Dobro je poznato da kljuĉnu ulogu u imunskom odgovoru na LPS ima receptor TNFR1 (tumor necrosis factor receptor-1), kao i da TNFR1-/- (tumor necrosis factor receptor-1 knockout) miševi pokazuju rezistenciju na endotoksiĉni šok ĉak i nakon tretmana letalnim dozama LPS-a. Dosadašnja istraţivanja su pokazala da LPS izaziva migraciju nekoliko ćelijskih tipova unutar slezine. MeĊutim, nema podataka o uticaju LPS-a na populaciju B i T limfocita koji se nalaze u crvenoj pulpi slezine miša. Nedavna studija istakla je znaĉaj hemokina CCL20 u brzoj akumulaciji B limfocita u slezini miša nakon sistemske administracije Nod1 agoniste, koji izaziva sistemsku inflamaciju in vivo. Cilj: Ispitivanje uticaja sistemske administracije LPS-a na broj B i T limfocita u crvenoj pulpi slezine miša, kao i na strukturnu organizaciju bele pulpe slezine, 24 h nakon tretmana. Osim toga, cilj ove studije bio je da ispita uticaj sistemske administracije LPS-a na ekspresiju gena za hemokine i proinflamatorne citokine u slezini miša 1 h, 2 h i 24 h nakon tretmana. Dodatni cilj bio je da se ispitaju potencijalne promene u ekspresiji hemokina CCL20 u slezini miša 2 h nakon sistemske administracije LPS-a, kao i da se odredi lokalizacija ćelija koje bi u ovim uslovima proizvodile CCL20. Materijal i metode: Eksperimenti su izvoĊeni na wild-type miševima soja C57BL/6 i TNFR1-/- miševima istog soja. Za ispitivanje uticaja sistemske administracije LPS-a na broj B i T limfocita u crvenoj pulpi slezine, kao i na strukturnu organizaciju bele pulpe slezine, formirane su ĉetiri grupe ţivotinja: netretirani wild-type miševi (n=5), tretirani wild-type miševi (n=5), netretirani TNFR1-/- miševi (n=5) i tretirani TNFR1-/- miševi (n=5). Tretman ţivotinja podrazumevao je ubrizgavanje u repnu venu LPS-a E. coli (soj 055:B5) rastvorenog u fosfatnom puferu, u dozi 2,5 μg/g telesne mase...sr
dc.description.abstractLipopolysaccharide (LPS) is a major component of the outer membrane of Gram negative bacteria. Recognition of LPS initiates intracellular signaling pathways which lead to gene transcription and release of proinflammatory mediators. It is well known that the key role in the immune response to LPS has the receptor TNFR1 (tumor necrosis factor receptor-1) and that TNFR1-/- (tumor necrosis factor receptor-1 knockout) mice show resistance to endotoxic shock even after treatment with lethal doses of LPS. Previous research has shown that LPS causes migration of several cell types within the spleen. However, there is no data on the effect of LPS on the population of B and T lymphocytes found in the red pulp of the mouse spleen. A recent study highlighted the importance of chemokine CCL20 in the rapid accumulation of B lymphocytes in the mouse spleen after systemic administration of Nod1 agonist, which induces systemic inflammation in vivo. Aim: Examination of the effects of systemic administration of LPS on the number of B and T lymphocytes in the red pulp of the mouse spleen, as well as on the structural organization of the white pulp of the spleen, 24 hours after treatment. In addition, the aim of this study was to examine the effect of systemic administration of LPS on the expression of genes for chemokines and proinflammatory cytokines in the mouse spleen 1 h, 2 h, and 24 h after treatment. An additional goal was to investigate the potential changes in the expression of chemokine CCL20 in the mouse spleen 2 h after systemic administration of LPS, and to determine the localization of cells that might produce CCL20 in these conditions. Material and methods: The experiments were performed on C57BL/6 wild-type and TNFR1-/- mice. In order to examine the effects of systemic administration of LPS on the number of B and T lymphocytes in the red pulp of the spleen, as well as on the structural organization of the splenic white pulp, four groups of animals were formed: untreated wild-type mice (n = 5), treated wild-type mice (n = 5), untreated TNFR1-/- mice (n = 5) and treated TNFR1-/- mice (n = 5)...en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Медицински факултетsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175005/RS//
dc.rightsopenAccessen
dc.sourceУниверзитет у Београдуsr
dc.subjectlimfocitsr
dc.subjectlymphocyteen
dc.subjectred pulpen
dc.subjectchemokineen
dc.subjectlipopolysaccharideen
dc.subjectspleenen
dc.subjectmouseen
dc.subjectcrvena pulpasr
dc.subjecthemokinsr
dc.subjectlipopolisaharidsr
dc.subjectslezinasr
dc.subjectmišsr
dc.titleUticaj sistemske administracije lipopolisaharida na strukturne, celularne i molekularne karakteristike slezine mišasr
dc.title.alternativeThe impact of systemic administration of lipopolysaccharide on structural, cellular and molecular characteristics of the mouse spleen.en
dc.typedoctoralThesis
dc.rights.licenseBY-NC-ND
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/9653/IzvestajKomisije18734.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/9652/Disertacija.pdf


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