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Behavioral effects of chronic administration of supraphysiological doses of androgenic anabolic steroids and program of exercise protocol in rats

dc.contributor.advisorRosić, Gvozden
dc.contributor.otherJakovljević, Vladimir Lj.
dc.contributor.otherMilovanović, Dragan
dc.contributor.otherMitrović, Dušan M.
dc.contributor.otherMitrović, Slobodanka
dc.creatorSelaković, Dragica V.
dc.date.accessioned2018-12-26T15:02:51Z
dc.date.available2018-12-26T15:02:51Z
dc.date.available2020-07-03T15:20:50Z
dc.date.issued2018-03-23
dc.identifier.urihttp://eteze.kg.ac.rs/application/showtheses?thesesId=5909
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/10495
dc.identifier.urihttps://fedorakg.kg.ac.rs/fedora/get/o:991/bdef:Content/download
dc.description.abstractUvod: U ovoj studiji su ispitivani bihevioralni efekti hronične primene suprafizioloških doza androgenih anaboličkih steroida (AAS) i programiranog trenažnog procesa kod pacova. Materijal i metode: Studija je sprovedena na 64 pacova Wistar albino soja, starosti 3 meseca, telesne mase 350-400 g, podeljenih u 6 grupa: kontrolna, grupa sa vežbanjem (V), nandrolon-dekanoat grupa (ND), kombinovana grupa za ND, testosteron-enantat grupa (TE) i kombinovana grupa za TE (V+TE). AAS su primenjivani u dozi od 20 mg/kg nedeljno, dok je protokol fizičke aktivnosti sprovođen u formi jednosatnog plivanja (5 dana nedeljno). Protokoli su trajali po šest nedelja, nakon čega su vršeni bihevioralni testovi, a potom su životinje žrtvovane u cilju dobijanja uzoraka za serološke i histološke analize. Rezultati: Rezultati bihevioralnog ispitivanja su pokazali da AAS dovode do ispoljavanje anksiogenog i prodepresantnog efekta, uz smanjenje kognitivnih funkcija i tolerancije bola. Hronični protokol fizičke aktivnosti je dovodio do suprotnih bihevioralnih manifestacija. Svi primenjeni protokoli su dovodili do povećanja serumskih koncentracija polnih hormona. Suprafiziološke doze AAS su uzrokovale smanjenje broja parvalbumin pozitivnih interneurona i povećanje broja receptora za androgene hormone u hipokampusu. Fizička aktivnost je dovodila do povećanja parvalbumin pozitivnih interneurona i estrogen α receptora, uz smanjenje receptora za androgene hormone u hipokampusu. Parametri za procenu oksidativnog oštećenja u tkivu hipokampusa su se povećavali nakon tretmana AAS i smanjivali nakon protokola fizičke aktivnosti. Zaključak: Anksiogeni i prodepresantni efekat, uz smanjenje kognitivnih funkcija, nakon primene suprafizioloških doza AAS je bio povezan sa smanjenjem broja parvalbumin pozitivnih interneurona i povećanjem broja receptora za androgene hormone u hipokampusu. Suprotni bihevioralni efekti dobijeni nakon hroničnog trenažnog procesa su bili udruženi sa istovremenim smanjenjem broja androgenih i povećanjem broja estrogen α receptora, uz značajno povećanje broja parvalbumin pozitivnih interneurona u hipokampusu.sr
dc.description.abstractIntroduction: In this study we evaluated behavioral effects of chronic administration of supraphysiological doses of androgenic anabolic steroids (AASs) and program of exercise protocol in rats. Material and methods: The study was performed on 64 Wistar albino rats, 3 months old, weighting 350-400 g, divided into 6 groups: control, exercise group (E), nandrolon-decanoate group (ND), combined group for ND, testosterone-enanthate group (TE), and combined group for TE. AASs were administered in a dose of 20 mg/kg weekly, exercise protocol was performed by means of 1 hour of swimming (5 days per week). All protocols lasted for six weeks, and were followed by behavioral testing, and then the animals were sacrificed in order to obtain the samples for serological and histological analyses. Results: The results of behavioral testing showed AASs-induced anxiogenic and prodepressant effect accompained with decline of cognitive functions and pain tolerance. Chronic exercise protocol resulted in the opposite behavioral manifestations. All applied protocols resulted in increase in sex hormones serum levels. Supraphysiological doses of AASs induced decrease the number of parvalbumin positive interneurons and increase in number of androgen receptors in hippocampus. Exercise protocol resulted in increased number of parvalbumin positive interneurons and estrogen α receptors, accompanied with decreased number of androgen receptors in hippocampus. Parameters for evaluation of oxidative damage in hippocampal tissue were elevated following AASs treatment, and decrease after exercise protocol. Conclusion: Anxiogenic and prodepressant effect, with decline in cognitive functions, following administration of supraphysiological doses of AASs was accompained with decreased number of parvalbumin positive interneurons and increased number of androgen receptors in hippocampus. The opposite behavioral effects achieved following exercise protocol were accompained with simultaneous decline in number of androgen and increase in number of estrogen α receptors, with significant augmentation of parvalbumin positive interneurons number in hippocampus.en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Крагујевцу, Факултет медицинских наукаsr
dc.rightsopenAccessen
dc.sourceУниверзитет у Крагујевцуsr
dc.subjectandrogeni anabolički steroidisr
dc.subjectandrogenic anabolic steroidsen
dc.subjectfizička aktivnostsr
dc.subjectbihevioralni testovisr
dc.subjectreceptori za polne hormonesr
dc.subjecthipokampussr
dc.subjectexerciseen
dc.subjectbehavioral testsen
dc.subjectsex hormones receptorsen
dc.subjecthippocampusen
dc.titleBihevioralni efekti hronične primene suprafizioloških doza androgenih anaboličkih steroida i programiranog trenažnog procesa kod pacovasr
dc.title.alternativeBehavioral effects of chronic administration of supraphysiological doses of androgenic anabolic steroids and program of exercise protocol in ratsen
dc.typedoctoralThesis
dc.rights.licenseBY-NC-ND
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/50352/Dragica_Selakovic_Medicina.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/50351/Disertacija.pdf


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