Disproteinemija i dislipoproteinemija tokom odgovora akutne faze kod pasa prirodno inficiranih sa Babesia canis
Disproteinemia and dislipoproteinemia during acute phase response in dogs naturally infected with Babesia canis
Author
Milanović, Zorana Z.Mentor
Kovačević Filipović, Milica
Committee members
Ivanovski, PetarMilanović, Svetlana

Beletić, Anđelo
Metadata
Show full item recordAbstract
Najveći broj ispitivanja kod ljudi i životinja ukazuje da tokom odgovora akutne
faze dolazi do pada albumina, a povećanja α i β-globulina, dok su rezultati vezani za
promenu koncentracije lipida i lipoproteina manje ispitivani. Ipak, poznato je da se kod
teških inflamacija najčešće javlja poremećaj reverznog transporta holesterola praćen
padom lipoproteina visoke gustine (engl. High density lipoprotein - HDL) i
apolipoproteina A-I (engl. Apolipoprotein A-I - ApoA-I) koji predsavlja njegov glavni
apoprotein. ApoA-I se zbog toga smatra negativnim proteinom akutne faze. Istovremeno
dolazi do porasta seruma amiloida A (engl. Serum amyloid A – SAA) koji takođe ima
funkciju apoproteina HDL-a. Detaljno ispitivanje promena pojedinih frakcija proteina i
lipoproteina kao i promene u apoproteinima u toku odgovora akutne faze kod pasa,
izazvan infekcijom sa B. canis nisu do sada opisane.
Ovo istraživanje je izvedeno na višku uzoraka krvi od 30 pasa prirodno
inficiranih sa B. canis i 15 zdravih kon...trolnih pasa. Određeni su hematološki i
biohemijski parametri krvi i seruma, potom je izvedena elektroforeza na agaroznom
gelu i analizirane su proteinske i lipoproteinske frakcije seruma. Uz primenu
poliakrilamid gradijent gel elektroforeze su dobijeni podaci o dominantnim dijametrima
lipoproteina seruma, a upotrebom imunoenzimskog testa (engl. Enizime linked
immunosorbent assay – ELISA) i radioimunoeseja (engl. Radioimmunoassay – RIA)
testa su određene koncentracije SAA i ApoA-I.
Najzastupljenije hematološke promene kod pasa akutno inficiranih sa B. canis su
bile trombocitopenija i leukopenija. Koncentracija ukupnih proteina i triglicerida nije
bila promenjena dok je koncentracija holesterola bila snižena. Disproteinemija se
ogledala u smanjenju koncentracije α1- globulinske frakcije, dok ostale frakcije nisu
bile promenjene. Dislipidemija se ogledala u smanjenom relativnom udelu α1-
lipoproteinske frakcije u ukupnim α-lipoproteinima. Osim toga pokazano je da se tokom
odgovora akutne faze povećava dijametar lipoproteina koji spadaju u HDL.
Koncentracija oba proteina akutne faze - SAA i ApoA-I je bila povećana.
Navedene promene nisu tipične za odgovor akutne faze te zbog toga smatramo
da odgovor akutne faze kod pasa inficiranih sa B. canis predstvlja jedinstven model za
ispitivanje promena u metabolizmu lipida i lipoproteina u uslovima kada postoji
paralelno povećanje ApoA-1 i SAA.
In humans and animals acute phase response (APR) is characterized by decrease
in albumines and increase in α i β-globulines. Changes in lipids and lipoproteins are less
known. During severe inflammatory events high density lipoproteins (HDL) and it's
major apoprotein apolipoprotein A-I (ApoA-I) are significantly decreased.
ApoA-I is considered as negative acute phase protein. Serum amyloid A (SAA)
which also has apoprotein function is increased. Detail examination of protein and
lipoprotein fractions changes during APR in B. canis infection in dogs were not done.
Present study was done on surplus material (blood and sera) from 30 dogs
naturally infected with B. canis and 15 healthy dogs. Biochemistry and hematology
parameters were determined, protein and lipoproteins were separated electrophoreticaly
on agarose gels, and ther dominant diameters were determined using polyacrylamide
gradient gel electrophoresys. Concentrations of SAA and ApoA-I were determined
using enzyme linked immunos...orbent assay (ELISA) and radioimmunoassay (RIA) tests.
Detected hematology changes in sick dogs were thrombocytopenia and
leucopenia. Total protein and triglyceride concentration were unchanged in sick dogs,
and total cholesterol and phospholipids were decreased.
Dysproteinemia was characterized by drop in α1-globuline fraction. Detected
dyslipidemia included decrease in relative portion of α1-lipoprotein in relation to total
α-lipoproteins. During APR, the dominant diameter of HDL in sick dogs was increased.
Concentration of SAA and ApoA-I was increased. Those changes are not typical for
APR. Based on this results we consider that APR in dogs infected with B. canis
represent unique model for examination of lipid and lipoprotein metabolism when there
is increase in both SAA and ApoA-I.
Faculty:
Универзитет у Београду, Факултет ветеринарске медицинеDate:
18-07-2018Projects:
- Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials (EU-621375)
- Antioxidative defense, differentiation and regeneration potential of tissue specific mesenchymal stem cells during ageing (RS-175061)