Show simple item record

High-valuable functional compounds from tomato by-products

dc.contributor.advisorĆetković, Gordana
dc.contributor.otherČanadanović-Brunet, Jasna
dc.contributor.otherĆetković, Gordana
dc.contributor.otherMandić, Anamarija
dc.creatorStajčić, Slađana
dc.date.accessioned2018-12-19T17:10:42Z
dc.date.available2018-12-19T17:10:42Z
dc.date.available2020-07-03T13:53:13Z
dc.date.issued2011-11-02
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/10375
dc.identifier.urihttps://www.cris.uns.ac.rs/DownloadFileServlet/Disertacijadisertacija.pdf?controlNumber=(BISIS)85034&fileName=disertacija.pdf&id=1063&source=NaRDuS&language=srsr
dc.identifier.urihttps://www.cris.uns.ac.rs/record.jsf?recordId=85034&source=NaRDuS&language=srsr
dc.identifier.urihttps://www.cris.uns.ac.rs/DownloadFileServlet/IzvestajKomisije152403657534597.pdf?controlNumber=(BISIS)85034&fileName=152403657534597.pdf&id=11174&source=NaRDuS&language=srsr
dc.description.abstractEkstrakcijom heksanom, a zatim 80% etanolom predhodno pripremljenog tropa od IZ odabranih genotipova Bačka, Knjaz, Novosadski niski, O2, Rutgers i Saint Pierre paradajza, dobijeni su heksanski i etanolni ekstrakti tropa. Sadržaj karotenoida u heksanskim i polifenolnih jedinjenja, flavonoida i askorbinske kiseline u etanolnim ekstraktima određen je spektrofotometrijskim metodama. Identifikacija i kvantifikacija pojedinih karotenoida u heksanskim i polifenolnih jedinjenja u etanolnim ekstraktima tropa paradajza izvedena je HPLC analizom. U ostacima nakon ekstrakcija tropa paradajza određen je sadržaj prehrambenih vlakana. Spektrofotometrijskim testovima određena je antiradikalska aktivnost na DPPH radikale i redukciona sposobnost dobijenih ekstrakata. ESR spektroskopijom ispitan je uticaj etanolnih ekstrakata na reaktivne hidroksil i superoksid anjon radikale. Pored toga, ispitana je i helirajuća sposobnost etanolnih ekstrakata. Antiradikalsko delovanje na DPPH radikale ostataka nakon ekstrakcija tropa paradajza radikale, takođe je utvrđeno. Ispitana je in vitro antiproliferativna aktivnost frakcija ekstrakata, njihovim delovanjem na rast tri histološki različite humane ćelijske linije: MCF-7 (adenokarcinom dojke), HeLa (epitelni karcinom cerviksa) i MRC-5 (fetalni fibroblastni karcinom pluća). Rezultati ispitivanja hemijskog sastava, antioksidativne i antiproliferativne aktivnosti ekstrakata, kao i ostataka nakon ekstracija tropa odabranih genotipova paradajza ukazuju na mogućnost iskorišćenja ovog sporednog proizvoda kao potencijalnog izvora prirodnih antioksidanata, koji bi našli primenu u prehrambenoj, farmaceutskoj i kozmetičkoj industriji.sr
dc.description.abstractTomato pomaces obtained from selected tomato genotypes (Bačka, Knjaz, Novosadski niski, O2, Rutgers and Saint Pierre) were extracted sequentially with hexane and 80% ethanol. The content of carotenoids i hexane and total polyphenolics, flavonoids and ascorbic acid in ethanolic extracts was determined by spectrophotometric methods. Identification and quantification of individual carotenoids in hexane and polyphenolics in ethanolic extracts was determined by HPLC analysis. The content of dietary fiber was determined in the residues after extraction of tomato pomaces. The scavenging activity on DPPH radicals and reducing power of the obtained extracts were determined spectrophotometrically. The influence of ethanolic extracts on reactive hydroxyl and superoxide anion radicals was examined by electron spin resonance (ESR) spectroscopy. In addition, the chelating ability of ethanolic extracts was investigated. Scavenging effect on DPPH radicals of the residue after extraction of tomato pomace, has also been established. Antiproliferative activity of investigated extracts was determined in vitro, testing their influence on the growth of three histologically different human cell lines: MCF-7 (breast adenocarcinoma), HeLa (cervix epithelioid carcinoma) and MRC-5 (fetal lung). Based on the results of chemical analysis, significant antioxidant and antiproliferative activity of the tomato waste extracts, as well as residues after extraction showed that tomato waste obtained from different tomato genotypes should be regarded as potential source of natural antioxidants, which can be used for various purposes in the food, pharmaceutical and cosmetic industry.en
dc.languagesr (latin script)
dc.publisherУниверзитет у Новом Саду, Технолошки факултетsr
dc.rightsopenAccessen
dc.sourceУниверзитет у Новом Садуsr
dc.subjectTrop paradajzasr
dc.subjectTomato pomaceen
dc.subjectantioksidativna aktivnostsr
dc.subjectantiproliferativna aktivnostsr
dc.subjectfenolna jedinjenjasr
dc.subjectkarotenoidisr
dc.subjectDPPHsr
dc.subjecthidroksil radikalisr
dc.subjectsuperoksidanjon radikalisr
dc.subjectHPLCsr
dc.subjectESRsr
dc.subjectantioxidant activityen
dc.subjectantiproliferative activityen
dc.subjectphenolic compoundsen
dc.subjectcarotenoidsen
dc.subjectDPPHen
dc.subjecthidroksil radicalsen
dc.subjectsuperoxide anion radicalsen
dc.subjectHPLCen
dc.subjectESRen
dc.titleVisokovredna funkcionalna jedinjenja iz sporednih proizvoda prerade paradajzasr
dc.title.alternativeHigh-valuable functional compounds from tomato by-productsen
dc.typedoctoralThesissr
dc.rights.licenseBY-NC-ND
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/39988/Disertacija.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/39989/IzvestajKomisije.pdf


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record