Funkcionalne i antioksidativne osobine tropa cvekle (Beta vulgaris)
Functional and antioxidant characteristics of beetroot pomace (Beta vulgaris)
Abstract
Etanolni ekstrakti tropa odabranih sorti cvekle (Detroit, Cardeal-F1, Egipatska, Bikor i Kestrel) prečišćeni su primenom ekstrakcije na čvrstoj fazi (SPE). Sadržaj ukupnih fenolnih jedinjenja, flavonoida i betalaina u prečišćenim ekstraktima određeni su spektrofotometrijskim metodama. HPLC analizom utvrđen je kvalitativni i kvantitativni sastav fenolnih jedinjenja i betalaina ekstrakata tropa odabranih sorti cvekle. ESR spektroskopijom ispitana je antiradikalska aktivnost ekstrakata topa cvekle na stabilne DPPH i reaktivne superoksid anjon i hidroksil radikale. Spektrofotometrijski je određena antioksidativna aktivnost na DPPH radikale i redukciona sposobnost po Oyaizu u ekstraktima odabranih sorti cvekle. Ispitana je in vitro antiproliferativna aktivnost frakcija ekstrakata, njihovim delovanjem na rast tri histološki različite humane ćelijske linije: MCF-7 (adenokarcinom dojke), HeLa (epitelni karcinom cerviksa)i MRC-5 (fetalni fibroblastni karcinom pluća). U završnoj fazi rada određe...
Beetroot (Detroit, Cardeal-F1, Egipatska, Bikor i Kestrel) pomace ethanol extracts were purified using solid phase extraction (SPE). Contents of total phenols, flavonoids and betalains in purified extracts were determined by spectrophotometric methods. HPLC analysis were used for quantitative and qualitative characterization of phenolic compounds and betalains in investigated extracts. ESR spectroscopy was used for investigation of antiradical activity of beetroot pomace extracts on stable DPPH and reactive superoxide anion and hydroxyl radicals. Antioxidant activity was determined spectrophotometrically on DPPH radicals and reducing power according to Oyaizu in the beetroot pomace extracts. Antiproliferative activity of investigated extracts was determined in vitro, testing their influence on the growth of three histologically different human cell lines: MCF-7 (breast adenocarcinoma), HeLa (cervix epithelioid carcinoma) and MRC-5 (fetal lung). Also, antimicrobial activity of beetroot ...