Morfološka, molekularna i patogena karakterizacija vrsta Diaporthe/Phomopsis na soji u Srbiji
Morphological, molecular and pathogenic characterization of species Diaporthe/Phomosis on soybean in Serbia
Doktorand
Petrović, Kristina P.Mentor
Ivanović, MirkoČlanovi komisije
Vidić, MilošKrstić, Branka
Đorđević, Vuk
Bulajić, Aleksandra
Metapodaci
Prikaz svih podataka o disertacijiSažetak
Endofitne vrste roda Diaporthe/Phomopsis imaju izuzetno širok spektar
domaćina i u biljkama mogu biti prisutne kao patogeni ili saprofiti. Na stablu i
semenu soje (Glycine max (L.) Merr.), vrste ovog roda prouzrokuju kompleks
oboljenja i spadaju u grupu značajnih parazita ove biljne vrste. Kao paraziti soje
opisane su vrste Diaporthe phaseolorum i Phomopsis longicolla. U okviru prvo
pomenute vrste razlikuju se tri varijeteta, i to: D. phaseolorum var. sojae, D.
phaseolorum var. caulivora i D. phaseolorum var. meridionalis. Iako su u
mnogobrojnim istraživanjima detaljno opisane morfološke i odgajivačke
karakteristike ovih patogena, njihova taksonomija još uvek nije u potpunosti
definisana. Najveća taksonomska nedoumica odnosi se na D. phaseolorum var.
sojae, jer je u okviru ovog varijeteta uočena izražena varijabilnost morfoloških
karakteristika. S obzirom da je morfološka varijabilnost D. phaseolorum var. sojae
uočena i kod izolata poreklom iz Srbije, ukazala se potreba za detaljnim
is...pitivanjem, kako bi se tačno utvrdile prisutne Diaporthe/Phomopsis vrste na soji
u Srbiji. Uporednim proučavanjem morfoloških, molekularnih i patogenih
karakteristika, identifikovano je ukupno sedam vrsta roda Diaporthe/Phomopsis.
Pored poznatih parazita, P. longicolla, D. phaseolorum var. sojae i D. phaseolorum
var. caulivora, na semenu soje su detektovane i vrste D. neotheicola, D. eres, D.
viticola i neidentifikovana grupa Phomopsis sp. 9. Identifikacija i filogenetsko
grupisanje Diaporthe/Phomopsis vrsta sprovedeno je na osnovu sekvenci ITS
regiona i dela LSU i TEF1-α gena. U cilju iznalaženja bržeg načina detekcije i
identifikacije, ITS sekvence proučavanih vrsta poslužile su za virtuelnu digestiju
restrikcionim enzimima AluI, RsaI, HhaI, MseI i StyI. S obzirom da su za svaku
proučavanu vrstu dobijeni različiti molekularni profili, konstatovano je da PCRRFLP
tehnika potencijalno može biti korisna metoda za brzu i pouzdanu
identifikaciju Diaporthe/Phomopsis vrsta koje se javljaju na soji. Proučavanjem
morfoloških karakteristika identifikovanih vrsta, utvrđeno je da se dimenzije
reproduktivnih organa preklapaju, ali i da veoma variraju između izolata unutar
jedne vrste, kao i unutar samog izolata. Na osnovu toga, izveden je zaključak da dimenzije reproduktivnih organa nisu pouzdani pokazatelji pri identifikaciji
Diaporthe/Phomopsis vrsta izolovanih iz soje...
Species of the genus Diaporthe/Phomopsis are endophytes on a very
wide range of hosts and can be present as pathogens or saprophytes. Species of
this genus cause a complex of diseases on the stem and seeds of soybean (Glycine
max (L.) Merr.) and therefore belong to a group of important parasites of this plant.
Diaporthe phaseolorum and Phomopsis longicolla were described as soybean
pathogens. The first species includes three varieties: D. phaseolorum var. sojae, D.
phaseolorum var. caulivora and D. phaseolorum var. meridionalis. Although several
studies have extensively described the morphological and culture characteristics of
these pathogens, their taxonomy has not fully been defined. The greatest
taxonomic confusion is related to D. phaseolorum var. sojae, because this variety
expressed variability of morphological characteristics. Since morphological
variability of D. phaseolorum var. sojae was also observed in isolates from Serbia,
there were needs for detailed examinations in ord...er to exactly determine which
Diaporthe/Phomopsis species are present on soybean in Serbia. By comparison of
morphological, molecular and pathogenic data seven Diaporthe/Phomopsis species
were identified. Besides the known parasites, D. neotheicola, D. eres, D. viticola and
unidentified group Phomopsis sp. 9 were also detected on soybean seeds.
Identification and phylogenetic grouping was performed based on sequences of
ITS region and parts of LSU and TEF1-α genes. In order to find faster ways of
detection and identification, ITS sequences of the studied species were used for the
virtual digestion with restriction enzymes AluI, RsaI, HhaI, MseI, and StyI. Since
different molecular profiles were generated for each studied species, it was
concluded that PCR-RFLP technique can potentially be a useful method for rapid
and reliable identification of Diaporthe/Phomopsis species that occur on soybean.
By studying morphological characteristics of the identified species, it was found
that the dimensions of reproductive organs overlapping, but also that they vary
between isolates within a species, as well as within the isolates. Based on this, it
was concluded that dimensions of reproductive organs are not reliable parameters
for identification of Diaporthe/Phomopsis species isolated from soybeans...
Fakultet:
Универзитет у Београду, Пољопривредни факултетDatum odbrane:
2012Projekti:
- Interdisciplinarni pristup stvaranju novih sorti soje i unapređenju tehnologije gajenja i dorade semena (RS-MESTD-Technological Development (TD or TR)-31022)