Farmakognozijsko ispitivanje podzemnih organa srpske velistike Ferula heuffelii Griseb. ex Heuffel (Apiaceae)

Abstract

Predmet doktorske disertacije je analiza hemijskog sastava i ispitivanje farmakološke aktivnosti izolata (etarskih ulja, hloroformskog i metanolnog ekstrakta i metabolita) podzemnih organa srpske velestike, Ferula heuffelii Griseb. ex Heuffel (Apiaceae). Srpska velestika je endemična vrsta koja naseljava klisure i stenovite terene istočne Srbije, jugozapadne Rumunije i zapadne Bugarske. Ova vrsta do sada nije ispitivana u pogledu hemijskog sastava i farmakološke aktivnosti. Etarska ulja podzemnih organa F. heuffelii izolovana su iz uzoraka prikupljenih na tri lokaliteta u Republici Srbiji: Sićevačkoj klisuri, Đerdapskoj klisuri i klisuri reke Pek. Etarska ulja su izolovana destilacijom vodenom parom i hemijski okarakterisana metodama GC-FID i GC-MS. Etarsko ulje izolovano iz podzemnih organa poreklom iz Sićevačke klisure (F1) bilo je bogato seskviterpenima (48,7%) i fenilpropanima (17,0%), a glavni sastojak bio je elemicin (12,5%). Etarska ulja izolovana iz podzemnih organa prikupljenih u Đerdapskoj klisuri (F2) i klisuri reke Pek (F3), karakterisao je visok sadržaj fenilpropanskih jedinjenja (56,0 i 29,7%, redom), pri čemu su elemicin (35,4 i 16,8%, redom) i miristicin (20,6 i 12,9%, redom), bili najzastupljenije komponente. Razlike između ispitivanih etarskih ulja bile su pre svega kvantitativne prirode (...)

The scope of this doctoral dissertation represents chemical analysis of underground parts of Ferula heuffelii Griseb. ex Heuffel (Apiaceae), and investigation of pharmacological activity of its isolates (chloroform and methanol extracts, essential oils and metabolites). Ferula heuffelii („srpska velestika“) is an endemic and rare perennial species which grows predominantly in gorges and stony places in Eastern Serbia, and locally in Southwestern Romania and Western Bulgaria. This plant has not been previously investigated for chemical composition and pharmacological activity. The essential oils of underground parts of F. heuffelii were isolated from the samples collected from three localities in Serbia: the Sićevo Gorge; the Djerdap Gorge and the gorge of the river Pek. The oils were isolated by hydrodistillation in a Clevenger-type apparatus and analyzed by GC-FID and GC-MS methods. The oil from underground parts obtained from the Sićevo gorge (F1) was rich in sesquiterpene hydrocarbons (48.7%) and phenylpropanoids (17%), and elemicin was the major constituent (12.5%). The oils isolated from underground parts collected in the Djerdap gorge (F2) and in the gorge of the river Pek (F3), were characterized by high content of phenylpropanoids (56.0 and 29.7%, respectively), with elemicin (35.4 and 16,8%, respectively) and myristicin (20.6 and 12,9%, respectively) being the main compounds. The differences in chemical composition between the essential oils were mainly quantitative (...)