Приказ основних података о дисертацији

Hemijska analiza i farmakološki efekti ekstrakata i soka ploda aronije, Aronia melanocarpa (Michx.) elliott

dc.contributor.advisorKitić, Dušanka
dc.contributor.otherCvetković, Tatjana
dc.contributor.otherBranković, Suzana
dc.contributor.otherJanković Veličković, Ljubinka
dc.contributor.otherŠavikin, Katarina
dc.creatorMilutinović, Milica
dc.date.accessioned2021-03-23T10:02:26Z
dc.date.available2021-03-23T10:02:26Z
dc.date.issued2020-07-16
dc.identifier.urihttp://eteze.ni.ac.rs/application/showtheses?thesesId=7834
dc.identifier.urihttps://fedorani.ni.ac.rs/fedora/get/o:1669/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70052&RID=17750537
dc.identifier.urihttps://nardus.mpn.gov.rs/handle/123456789/18145
dc.descriptionThe aim of the doctoral dissertation is to define the chemical composition and to examine the potential pharmacological effects of the chokeberry (Aronia melanocarpa (Michx.) Elliott) extracts and juice. In recent years, chokeberry fruits have been in the focus of scientific research because of their numerous pharmacological effects due to the high content of phenolic compounds, especially anthocyanins. For the purpose of the experiment, three different samples were prepared, chokeberry juice (AS), fruit extract (AE), as well as a waste extract (AOE). A maceration method with 50% ethanol was used for the plant material extraction. After the extraction, the samples were lyophilized and chemically characterized using high-performance liquid chromatography (HPLC). The antioxidant activity was evaluated using two in vitro methods, DPPH (1,1-diphenyl-2-picrylhydrazyl) and β-carotene-linoleic acid assay. The antimicrobial activity of the samples was tested against bacterial and fungal laboratory strains using a micro-dilution method. The spasmolytic activity of chokeberry extracts and juice were examined on spontaneous and induced contractions of the isolated ileum, while vasorelaxant effects were evaluated on the isolated rat aorta. The protective effects of the chokeberry preparation in the cisplatin-induced rat kidney and liver damage were evaluated by the values of biochemical, antioxidant and inflammatory parameters in blood and tissues, as well as by the histopathological analyses of the kidney and liver tissue. The highest content of phenolic compounds and anthocyanins was determined in AOE. HPLC analysis showed that cyanidin-3-O-galactoside was the most abundant compound. Chokeberry extracts and juice were potent antioxidants in vitro. The investigated preparations exhibited inhibitory activity on the growth of the used microbial strains. Gram (+) bacteria were slightly more sensitive than Gram (-) bacteria and fungus. The preparations dose-dependently inhibited spontaneous and induced contractions of the rat ileum and aorta, with a significant difference in the effects of extracts and juice due to their chemical composition. The chokeberry preparations could alleviate the kidney and liver damage caused by the nephrotoxicity and hepatotoxicity of cisplatin, while the protective effects resulted from their antioxidant and anti-inflammatory activity in plasma and tissues. The results of the research have scientifically confirmed the pharmacological use of the chokeberry fruit and its potential application in modern phytotherapy. These pharmacological effects need to be confirmed by relevant clinical studies.en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Нишу, Медицински факултетsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41018/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46013/RS//
dc.rightsopenAccessen
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceУниверзитет у Нишуsr
dc.subjectAronia melanocarpa (Michx.) Elliottsr
dc.subjectAronia melanocarpa (Michx.) Elliotten
dc.subjectextractsen
dc.subjectjuiceen
dc.subjectphenolsen
dc.subjectanthocyaninsen
dc.subjectproanthocyanidinsen
dc.subjectflavonoidsen
dc.subjectantioxidant activityen
dc.subjectantimicrobial activityen
dc.subjectspasmolytic activityen
dc.subjectvasorelaxant activityen
dc.subjectnephroprotective activityen
dc.subjecthepatoprotective activityen
dc.subjectekstraktisr
dc.subjectsoksr
dc.subjectfenolisr
dc.subjectantocijanisr
dc.subjectproantocijanidinisr
dc.subjectflavonoidisr
dc.subjectantioksidativna aktivnostsr
dc.subjectantimikrobna aktivnostsr
dc.subjectspazmolitička aktivnostsr
dc.subjectvazorelaksantna aktivnostsr
dc.subjectnefroprotektivna aktivnostsr
dc.subjecthepatoprotektivna aktivnostsr
dc.titleHemijska analiza i farmakološki efekti ekstrakata i soka ploda aronije, Aronia melanocarpa (Michx.) elliottsr
dc.typePhD thesis
dc.rights.licenseBY-NC-ND
dcterms.abstractКитић, Душанка; Бранковић, Сузана; Цветковић, Татјана; Шавикин, Катарина; Јанковић Величковић, Љубинка; Милутиновић, Милица;
dc.identifier.fulltexthttps://nardus.mpn.gov.rs/bitstream/id/70352/Disertacija.pdf
dc.identifier.fulltexthttps://nardus.mpn.gov.rs/bitstream/id/70353/Milutinovic_Milica_S.pdf
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_nardus_18145


Документи за докторску дисертацију

Thumbnail
Име:
Disertacija.pdf
Величина:
8.095Mb
Формат:
PDF
Отварање
Thumbnail
Име:
Milutinovic_Milica_S.pdf
Величина:
1.905Mb
Формат:
PDF
Отварање

Ова дисертација се појављује у следећим колекцијама

Приказ основних података о дисертацији