Adhesive joints offer many advantages compared to the traditional joining techniques in metallic structures. However, the lack of unified design standards and procedures limit their wider use in civil engineering industry. For the purpose of standardization, extensive experimental work has to be done on investigating their behavior under the service conditions. Besides the influence of the joint geometry, the manufacturing process and the temperature and humidity effects, type of the loading has the greatest influence on the behavior of adhesive joints. Main goal of this dissertation was to investigate behavior of adhesive joints in metallic structures under static and cyclic loading, using modern experimental and numerical methods. In order to achieve this the selection of proper adherent surface preparation procedure was necessary. Experimental investigation of the behavior of adhesive joints subjected to static and cyclic loading was conducted on two types of adhesive joints: