Detekcija mehanizama rezistencije na karbapeneme kod enterobakterija i vrste Pseudomonas aeruginosa

The aims of the research: to examine the presence of genes which encode carbapenemases among enterobacteria and Pseudomonas aeruginosa isolates with reduced sensitivity to carbapenems by using the polymerase chain reaction (PCR); to determine the most common mechanisms of resistance to carbapenems using phenotypic methods. To evaluate the phenotypic methods in relation to PCR as a reference method, and determine the most common phenotypes. Methods: 107 isolates from the Enterobacteriaceae family and 75 isolates of the species Pseudomonas aeruginosa were examined. Antimicrobial susceptibility was determined by the Kirby-Bauer disc-diffusion method and the automated Vitek2 system according to the recommendations of the Institute for Clinical and Laboratory Standards (CLSI). Using PCR and phenotypic methods, 56 enterobacterial isolates and 14 isolates of P. aeruginosa were tested. The results of the phenotypic tests were validated by a comparison with genotypic data and expressed

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Ključne reči:

Rezistencija na karbapeneme, genotipska detekcija, fenotipska detekcija, Enterobacteriaceae, Pseudomonas aeruginos; Carbapenem resistance, genotypic detection, phenotypic detection, Enterobacteriaceae, Pseudomonas aeruginosa