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Biocompatibility of functionalized carbon fibers implanted in rabbit subcutaneous and muscle tissues

dc.contributor.advisorKrstić, Nikola
dc.contributor.otherTodorović, Vera
dc.contributor.otherVasić, Jugoslav
dc.contributor.otherMarković, Danica
dc.contributor.otherDaković, Marko
dc.creatorProkić, Bogomir Bolka B.
dc.date.accessioned2017-12-12T15:45:06Z
dc.date.available2017-12-12T15:45:06Z
dc.date.issued2017-09-28
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=5420
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:16797/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=49634831
dc.identifier.urihttp://nardus.mpn.gov.rs/123456789/8940
dc.description.abstractU ovoj doktorskoj disertaciji upotrebljena su dobro okarakterisana komercijalna visokočvrsta karbonska vlakna (KV) marke Torayca (T300B, 6000-50B, No 2610622), debljine 4-6 m. Ova vlakna funkcionalizovana su tretiranjem HNO3 i KOH, što je značajno izmenilo sadržaj površinskih grupa. Biokompatibilnost tako funkcionalizovanih karbonskih vlakana ispitivana je na eksperimentalnim životinjama (kunići, varijetet činčila), a procenjivana je in vitro, na ćelijskim kulturama (fibroblastvoj i makrofagnoj), in vivo aplikacijom vlakana u potkožno tkivo i m. longissimus dorsi i primenom nuklearne magnetne rezonance (NMR). Rezultati ove doktorske disertacije pokazali su da ne postoji razlika u stepenu citotoksičnosti između modifikovanih i standardnih KV, koja je u ovom eksperimentu prvo procenjivana na osnovu testa vijabilnosti ćelija (kalorimetrijski MTT) i testa ćelijske adhezije (trodimenzionalna / 3D kultivacija ćelija u Matrigelu) na humanim fibroblastima kontinuirane ćelijske linije MRC-5. Međutim, ova istraživanja dopunjena su testovima na kulturi humane makrofagne linije U937, u kojima je procenjivana vijabilnost ćelija, internalizacija vlakana (fagocitoza) i produkcija proinflamatornih citokina, IL-1, IL6, IL-8 i TNF-. Ćelijska vijabilnost bila je nenarušena u kulturama humane makrofagne linije U937 ukoliko su se dodavala KV u koncentaciji od 125 g/ ml. Međutim, dodavanjem KV u koncentraciji od 250 g/ml ili 500 g/ml, dolazilo je do značajnog dozno-zavisnog linearnog smanjenja vijabilnosti ćelija kod sve tri primenjene grupe KV, i to naročito kod dodavanja baznih KV. Znatno veći broj makrofagnih ćelija fagocitovao je KV trerira bazom, u odnosu na fagocitozu standardnih i kiselih KV. Postojala je povećana koncentracija proinflamatornih citokina TNF- i IL-1 u supernatantima kulture humanih makrofaga linije U937 nakon dodavanja standardnih ili kiselih KV u koncentraciji od 125 g/ml, dok je dodavanje baznih KV u istoj koncentraciji uslovilo smanjenu produkciju ovih citokina. U istoj koncentraciji, standardna KV su uslovila značajno smanjenja sekrecije IL-8, a kisela i bazna KV izrazito povećanje sekrecije IL-6 i IL-8. Pri tome, vlakna tretirana kiselinom bila su snažniji stimulator sekrecije IL-8 iz makrofaga u odnosu na vlakna tretirana bazom...sr
dc.description.abstractIn this doctoral dissertation, it was used the well-characterized commercial high strenght carbon fibers (CF) Torayca (T300B, 6000-50B, No 2610622), 4-6 m thick. These fibers were functionalized by treatment with HNO3 and KOH, which significantly changed the content of the surface groups. Biocompatibility of functionalized carbon fibers was tested in vivo by implanting fibers in the subcutaneous tissue and m. longissimus dorsi of the experimental animals (rabbits, chinchilla variety), than followed with nuclear magnetic resonance (NMR), and assessed in vitro on cell cultures (fibroblast and macrophage). The results of this doctoral dissertation showed that there is no difference in the degree of the cytotoxicity level between modified and standard CFs, which was in this experiment evaluated first basing on the cell viability test (calorimetric MTT) and cell adhesion test (three-dimensional / 3D cell cultivation in Matrigel) on human fibroblasts of the continuous MRC-5 cell line. However, these studies were supplemented with tests on the culture of the human macrophage line U937, in which cell viability was assessed, than internalization of fibers (phagocytosis) and production of proinflammatory cytokines, IL-1α, IL6, IL-8 and TNF-α. When CFs were added at concentration of 125 μg / ml, cell viability was uninhabited in the culture of the human macrophage line U937. By adding CFs at concentration of 250 μg / ml or 500 μg / ml, there was a significant dose-dependent linear decrease in cell viability by all three applied CF groups, especially in case of adding base CFs. Significantly higher number CFs treated with base was pagocytosed by macrophage cells, compared to the phagocytosis of standard and acidic CFs. There was an increased concentration of proinflammatory cytokines TNF-α and IL-1β in human macrophage culture U937 line supernatants after adding of the standard or acidic CFs at concentration of 125 μg / ml, while the adding of the base CFs in the same concentration caused a reduced production of these cytokines. At the same concentration, standard CFs resulted in significant reductions in IL-8 secretion, while acid and base CFs pronouncedly increased secretion of IL-6 and IL-8 secretion. In addition, the acid-treated fibers were stronger stimulant of IL-8 secretion from macrophages, compared to the base-treated fibers. Standard CFs at the concentration of 250 μg / ml decreased IL-1β secretion, and base line increased...en
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dc.languagesr
dc.publisherУниверзитет у Београду, Факултет ветеринарске медицинеsr
dc.rightsAutorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
dc.sourceУниверзитет у Београдуsr
dc.subjectfunkcionalizovana karbonska vlakna, biokompatibilnost, zamorac, meka tkivasr
dc.subjectfunctionalized carbon fibers, biocompatibility, rabbits, soft tissueen
dc.titleIspitivanje biokompatibilnosti funkcionalizovanih karbonskih vlakana u potkožnom i mišićnom tkivu kunićasr
dc.title.alternativeBiocompatibility of functionalized carbon fibers implanted in rabbit subcutaneous and muscle tissuesen
dc.typePhD thesis
dcterms.abstractКрстић, Никола; Тодоровић, Вера; Васић, Југослав; Марковић, Даница; Даковић, Марко; Прокић, Богомир Болка Б.; Испитивање биокомпатибилности функционализованих карбонских влакана у поткожном и мишићном ткиву кунића; Испитивање биокомпатибилности функционализованих карбонских влакана у поткожном и мишићном ткиву кунића;


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