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Генотипизација, резистенција на антибиотике и формирање биофилма клиничких изолата Stenotrophomonas maltophilia из Србије

dc.contributor.advisorLozo, Jelena
dc.contributor.otherJovčić, Branko
dc.contributor.otherKojić, Milan
dc.contributor.otherLozo, Jelena
dc.creatorMadi, Haowa
dc.date.accessioned2017-11-17T15:27:02Z
dc.date.available2017-11-17T15:27:02Z
dc.date.available2020-07-03T08:05:24Z
dc.date.issued2017-09-08
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=5352
dc.identifier.urihttps://nardus.mpn.gov.rs/handle/123456789/8881
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:16661/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025175986
dc.description.abstractStenotrophomonas maltophilia is an environmental bacterium and an opportunistic pathogen usually associated with healthcare-associated infections, which has recently been recognized as a global multidrug resistant organism. The aim of this study was genotyping and physiological characterization of S. maltophilia collected during the routine health care at The Institute for Mother and Child Health Care of Serbia “Dr Vukan Čupić“. It is the large, tertiary care pediatric hospital in Belgrade, Serbia, hosting the national reference cystic fibrosis (CF) center for pediatric and adult patients. We characterized 88 S. maltophilia strains, 42 strains of cystic fibrosis (CF) and 46 strains of non-cystic fibrosis (non-CF) origin isolated from 2013 to 2015 in order to investigate their genetic relatedness and phenotypic traits. Genotyping was performed using sequencing of 16S rRNA gene, Pulse Field Gel Electrophoresis (PFGE) and Multi locus sequencing typing (MLST) analysis. Sensitivity to five relevant antimicrobial agents was determined, namely trimethoprim/sulfamethoxazole (TMP/SMX), chloramphenicol, ciprofloxacin, levofloxacin and tetracycline. Surface characteristics, motility, biofilm formation and adhesion to mucin were tested in all strains. In addition, influence of different factors (temperature, pH, agitation and CO2) on biofilm formation, kinetics of selected biofilm producers and effect of TMP/SMX on formed biofilm were analyzed. Statistical approach was used to determine correlations between obtained results. All analyzed clinical isolates belong to S. maltophilia species with identity ranging from 95% to 99% with S. maltophilia strains from the NCBI database what was confirmed by sequencing of PCR products of amplified 16S rRNA gene...en
dc.formatapplication/pdf
dc.languageen
dc.publisherУниверзитет у Београду, Биолошки факултетsr
dc.rightsopenAccessen
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceУниверзитет у Београдуsr
dc.subjectStenotrophomonas maltophiliasr
dc.subjectStenotrophomonas maltophiliaen
dc.subjecthealthcare-associated infectionssr
dc.subjecttrimethoprim–sulfamethoxazolesr
dc.subjectbiofilmsr
dc.subjectPFGEsr
dc.subjectMLSTsr
dc.subjectнозокомијалнa инфекцијаen
dc.subjectтриметоприм-сулфаметаксазолen
dc.subjectbiofilmen
dc.subjectPFGEen
dc.subjectMLSTen
dc.titleGenotyping, antibiotic resistance and biofilm formation of Stenotrophomonas maltophilia clinical isolates from Serbiaen
dc.title.alternativeГенотипизација, резистенција на антибиотике и формирање биофилма клиничких изолата Stenotrophomonas maltophilia из Србијеsr
dc.typedoctoralThesisen
dc.rights.licenseBY-NC-ND
dcterms.abstractЛозо, Јелена; Јовчић, Бранко; Којић, Милан; Лозо, Јелена; Мади, Хаоwа;
dc.identifier.fulltexthttps://nardus.mpn.gov.rs/bitstream/id/1056/Disertacija.pdf
dc.identifier.fulltexthttps://nardus.mpn.gov.rs/bitstream/id/1057/IzvestajKomisije15096.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/1056/Disertacija.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/1057/IzvestajKomisije15096.pdf
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_nardus_8881


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