Uticaj ekstrakata lista zelenog čaja i ploda borovnice na tok akutne bubrežne insuficijencije pacova izazvane gentamicinom

Abstract

We assessed effects of bilberry and green tea diet in rat model of gentamicininduced nephrotoxicity by monitoring functional and morphological changes in kidneys. The study was conducted on six groups of rats: control group (C), treated with only gentamicin (GM), treated with only green tea (GT), treated with only bilberry (B), treatedwith both gentamicin and green tea (GT+GM) and treated with both gentamicin and bilberry (B+GM). Kidney function, oxidant and antioxidant parameters of renal tissue, as well as histopathological studies were assessed. Morphometric analysis was used to quantify these histopathological changes. Gentamicin caused significant elevations in serum creatinine and urea and oxidative stress parameters (MDA and AOPP), while antioxidative enzyme catalase was significantly decreased. Histological sections of kidneys in GM group revealed necrosis of proximal tubules, vacuolation of cytoplasm and massive mononuclear inflammatory infiltrates in interstitium. Coadministration of green tea and bilberry with gentamicin histologically showed renoprotective effect. Histological results were confirmed and quantified by morphometric analysis. Also in GT+GM and B+GM group we measured ameliorated parameters of renal functions and antioxidative defense. Regenerative potential of green tea and bilberry after renal injury induced by gentamicin could be explained through the decrease of oxidative stress and lipid peroxidation. Green tea and bilberry are both natural antioxidants, and could be beneficial in the reduction of oxidative stress and changes caused by it primarily in renal tubules and interstitium.