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Immunophenotypic characterization of acute myeloid leukemia in adults and its role in the diagnosis,monitoring, and prognosis of the disease

dc.contributor.advisorSuvajdžić-Vuković, Nada
dc.contributor.otherBožić-Nedeljković, Biljana
dc.contributor.otherTošić, Nataša
dc.contributor.otherSuvajdžić-Vuković, Nada
dc.contributor.otherBožić-Nedeljković, Biljana
dc.creatorKraguljac-Kurtović, Nada J.
dc.date.accessioned2017-04-10T13:53:28Z
dc.date.available2017-04-10T13:53:28Z
dc.date.issued2016-09-29
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=4732
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:14986/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025142962
dc.identifier.urihttp://nardus.mpn.gov.rs/123456789/7832
dc.description.abstractU tezi su ispitivani savremeni aspekti primene imunofenotipizacije multiparametarskom protočnom citofluorimetrijom (IMPC) u dijagnostici i praćenju AML. Ispitivanjem je obuhvaćeno 320 odraslih bolesnika sa de novo AML, od kojih je 294 uključeno u retrospektivno ispitivanje (dijagnoza, klasifikacija i prognoza), dok je ostalih 26 bolesnika bilo uključeno u prospektivno ispitivanje (praćenje minimalne rezidualne bolesti - MRB). Bolesnici su klasifikovani kao AML-neklasifikovana (48%), AML sa rekurentnim genetskim poremećajima (37,4%) i AML sa znacima mijelodisplazije (14,6%). IPCM omogućava postavljanje dg AML analizom ks kod 98% bolesnika. Ispitivanjem 44 različita hLDM utvrđeno je da je njihova ekspresija na blastima deregulisana, o čemu govore aberacije u njihovoj ekspresiji kod svakog bolesnika. Heterogen ćelijski sastav populacije leukemijskih ćelija (leukemijski blasti i prekursori) utvrđen je kod 55% bolesnika sa AML. Imunološka i citomorfološka klasfikacija AML su saglasne kod 73% bolesnika, odnosno imunološka i SZO klasifikacija kod 68% bolesnika. Ispitivanje MRB sprovedeno je primenom jedne (42%) ili dve (58%) kombinacije IFSL po bolesniku. Primenom IMPC, pokazana je visoka učestalost MRB kod naših bolesnika sa AML posle lečenja indukcionom (69%) odnosno konsolidacionom terapijom (50%). Nivo MRB u ks bolesnika ≥0,1% NĆ posle indukcione terapije, svrstava bolesnike u grupu visokog rizika za razvoj relapsa bolesti. Ispitivanje prognostičkog značaja hLDM kod bolesnika sa AML, pokazalo je značajnu vezu između rane monocitne diferencijacije leukemijskih ćelija, ekspresije CD22 molekula i pojave rane smrti i (p<0,05), odnosno niže incidence kompletne remisije (p<0,05)...sr
dc.description.abstractIn the thesis are examined modern aspects of application immunophenotyping and multiparameter flow cytometry (IMPC) in the diagnosis and monitoring of AML. The study included 320 adult patients with de novo AML, of which 294 included in the retrospective study (diagnosis, classification and prognosis), while the other 26 patients were included in a prospective study (minimal residual disease - MRD). Patients were classified as AML-unclassified (48%), AML with recurrent genetic abnormalities (37.4%) and AML with signs of myelodysplasia (14.6%). IPCM allows setting dg AML by analysis of bone marrow (bm) in 98% of patients. By examining of 44 different HLDM, it was found that its expression is deregulated on the blasts, whereas at least one type of immunophenotypic aberrations was found per patient. Heterogeneous cellular composition of the population of leukemic cells (leukemic blasts and precursors) was found in 55% of patients with AML. Immunological and cytomorphological classification of AML agree with 73% of patients, respectively immunological and WHO classification in 68% of patients. MRD trial was conducted by one (42%) or two (58%) combination of IFSL per patient. By applying the IMPC, a high incidence of MRD was detected in our patients with AML, after induction (69%) and/or consolidation therapy (50%). The level of MRD in bm of patients ≥0,1% NC after induction therapy, classified patients in the high risk group for the development of relapse. The prognostic significance of HLDM in patients with AML, showed a significant association between early monocytic differentiation of leukemia cells, the expression of CD22 molecule and the appearance of early death (p<0.05), and lower incidence of complete remission (p<0.05)...en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Биолошки факултетsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41004/RS//
dc.rightsAutorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
dc.sourceУниверзитет у Београдуsr
dc.subjectAMLsr
dc.subjectAMLen
dc.subjectimunofenotipizacijasr
dc.subjectprotočna citofluorimetrijasr
dc.subjecthLDMsr
dc.subjectdijagnozasr
dc.subjectminimalna rezidualna bolestsr
dc.subjectprognozasr
dc.subjectimmunophenotypingen
dc.subjectflow cytometryen
dc.subjectHLDMen
dc.subjectdiagnosisen
dc.subjectminimal residual diseaseen
dc.subjectprognosisen
dc.titleImunofenotipska karakterizacija ćelija akutne mijeloidne leukemije kod odraslih i njena uloga u dijagnozi, praćenju i prognozi bolestisr
dc.title.alternativeImmunophenotypic characterization of acute myeloid leukemia in adults and its role in the diagnosis,monitoring, and prognosis of the diseaseen
dc.typePhD thesis
dcterms.abstractСувајджић-Вуковић, Нада; Божић-Недељковић, Биљана; Сувајджић-Вуковић, Нада; Тошић, Наташа; Божић-Недељковић, Биљана; Крагуљац-Куртовић, Нада Ј.; Имунофенотипска карактеризација ћелија акутне мијелоидне леукемије код одраслих и њена улога у дијагнози, праћењу и прогнози болести; Имунофенотипска карактеризација ћелија акутне мијелоидне леукемије код одраслих и њена улога у дијагнози, праћењу и прогнози болести;


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