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The influence of silver, copper and zinc ions on the properties of bioceramic materials based on calcium phosphate

dc.contributor.advisorJanaćković, Đorđe
dc.contributor.otherPetrović, Rada
dc.contributor.otherDimitrijević-Branković, Suzana
dc.contributor.otherKojić, Vesna
dc.creatorRadovanović, Željko M.
dc.date.accessioned2017-02-27T10:10:27Z
dc.date.available2017-02-27T10:10:27Z
dc.date.issued2016-09-27
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=4621
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:14756/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48698383
dc.identifier.urihttp://nardus.mpn.gov.rs/123456789/7657
dc.description.abstractKalcijum-fosfati čine neorganski deo kostiju i zuba sisara. Ova dobro poznata činjenica je ohrabrila istraživače da tokom prethodnih godina primene sintetisani kalcijum-hidroksiapatit (HAp, Ca10(PO4)6(OH)2) i kalcijum-fosfat (TCP, Ca3(PO4)2), α- i β-TCP faze, kao implantne materijale. Njihova sličnost sa neorganskim delom kostiju je potvrđena, a posebno je istaknuta njihova biokompatibilnost i osteokonduktivnost i dodatno bioaktivnost HAp-a i bioresorpcija TCP-a. Dvofazne forme (BCP): HAp/β-TCP i HAp/α-TCP, kao i trifazna forma HAp/β-TCP/α-TCP, pokazale su se kao bolji biomaterijali nego pojedinačne faze i posebno su interesantne za dalja istraživanja. U cilju dobijanja biomaterijala sa antimikrobnim efektom, prahovi HAp-a dopirani jonima Ag+, Cu2+ ili Zn2+ su sintetisani hidrotermalnom metodom pri čemu je odnos Ca/P iznosio 1,67. U sintezama molski udeo dopanata iznosio je 0,2, odnosno 0,4 mol% sa ciljem da se dobiju prahovi sa dobrom antimikrobnom aktivnošću uz očuvanje biokompatibilnosti. Dopirani prahovi HAp-a su kalcinisani na 1200 °C tokom 2 h sa namerom da se ispita uticaj dopiranja na stvaranje dvofaznih prahova HAp/α-TCP, kao i antimikrobna aktivnost i biokompatibilnost tako dobijenih prahova. Za analizu svih prahova, nedopiranih i dopiranih HAp i HAp/α-TCP, korišćeni su visokorezolucioni skenirajući elektronski mikroskop (FESEM), rendgenska difrakcija (XRD), infracrvena spektroskopija sa Furijeovom transformacijom (FTIR), atomska apsorpciona spektroskopija (AAS) i energetska disperziona spektroskopija (EDS). Termička analiza sintetisanih prahova je sprovedena u cilju određivanja faznih transformacija tokom zagrevanja do 1200 °C. Antibakterijska aktivnost prahova ispitana je u in vitro uslovima u odnosu na mikroorganizme: Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa) i Candida albicans (C. albicans). Svi prahovi su pokazali dobru antimikrobnu aktivnost, s tim da su prahovi dopiranih HAp/α-TCP dali malo bolje rezultate u odnosu na sve patogene mikroorganizme nego dopirani HAp prahovi. In vitro testovi biokompatibilnosti, kolorimetrijski test sa tetrazolijum solima (MTT) i test odbacivanja boje (DET), su korišćeni da bi se procenila biokompatibilnost dopiranih HAp/α-TCP prahova sa MRC-5 humanim fibroblastnim ćelijama. Ovi testovi su potvrdili da dati prahovi nemaju citotoksični efekat. Posebno dobru biokompatibilnost su pokazali prahovi HAp/α-TCP dopirani nižim udelom jona Ag+, Cu2+ i Zn2+. Na osnovu testova antimikrobne aktivnosti i biokompatibilnosti zaključeno je da su dopirani HAp/α-TCP prahovi obećavajući materijal za korišćenje u rekonstruktivnoj hirurgiji kostiju...sr
dc.description.abstractCalcium phosphates constitute the inorganic part of bones and teeth of mammals. This fact is well known and has encouraged researchers to apply hydroxyapatite (HAp, Ca10(PO4)6(OH)2) and tricalcium phosphate (TCP, Ca3(PO4)2), both α- and β-TCP, over the past years as implant materials. Their similarity to the inorganic part of hard tissues has been confirmed, highlighting their biocompatibility and osteoconductivity and, in addition, the bioactivity of HAp and the bioresorbability of TCP. The biphasic forms (BCP): HAp/β-TCP and HAp/α-TCP and the triphasic form HAp/β-TCP/α-TCP, which were proved to be even better biomaterial than single phases, are of particular interest. Hydroxyapatite (HAp) powders doped with Ag+, Cu2+ or Zn2+ were synthesized by a hydrothermal method, at a Ca/P ratio of 1.67, in order to obtain biomaterial with an antimicrobial effect. The synthesis was performed with two contents of dopant (0.2 or 0.4 mol %) by considering both the antimicrobial activities and biocompatibility of the powders. The doped HAp was annealed at 1200 °C for 2 h with the intention of investigating the influence of doping on the creation of biphasic HAp/α-TCP and determining the antimicrobial activity and biocompatibility of the obtained biphasic powders. Analyses of all powders, undoped and doped HAp and HAp/α-TCP, were performed by Field emission scanning electron microscopy (FESEM), X-Ray diffraction (XRD), Atomic absorption spectroscopy (AAS) and Energy-dispersive X-Ray spectroscopy (EDS). Thermal analysis of the synthesized powders was performed to evaluate the phase transformations during heating up to 1200 °C. The in vitro antibacterial activities of the powders were evaluated against the pathogenic microorganisms: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. All powders showed good antimicrobial activity but generally the powders of doped HAp/α-TCP demonstrated better results against all pathogenic microorganisms than the powders of doped HAp. In vitro biocompatibility tests, MTT and DET, were used to evaluate the biocompatibility of doped HAp/α-TCP with MRC-5 human fibroblast cells. These tests confirmed that the powders did not have a cytotoxic effect. The HAp/α-TCP powders doped with the lower content of Ag+, Cu2+ and Zn2+ ions showed especially good biocompatibility. The antimicrobial and biocompatibility tests recommend the doped HAp/α-TCP as promising material for use in reconstructive surgery of bones...en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Технолошко-металуршки факултетsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45019/RS//
dc.rightsAutorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
dc.sourceУниверзитет у Београдуsr
dc.subjecthidroksiapatitsr
dc.subjecthydroxyapatiteen
dc.subjectdopingen
dc.subjectcalcium phosphateen
dc.subjectantimicrobial activityen
dc.subjectbiocompatibilityen
dc.subjectsinteringen
dc.subjectbioceramicsen
dc.subjectscaffolden
dc.subjectmechanical propertiesen
dc.subjectdopiranjesr
dc.subjectkalcijum-fosfatsr
dc.subjectantimikrobna aktivnostsr
dc.subjectbiokompatibilnostsr
dc.subjectsinterovanjesr
dc.subjectbiokeramikasr
dc.subjectskafoldisr
dc.subjectmehanička svojstvasr
dc.titleUticaj jona srebra, bakra i cinka na svojstva biokeramičkih materijala na bazi kalcijum-hidroksiapatita i kalcijum-fosfatasr
dc.title.alternativeThe influence of silver, copper and zinc ions on the properties of bioceramic materials based on calcium phosphateen
dc.typePhD thesis
dcterms.abstractЈанаћковић, Ђорђе; Петровић, Рада; Димитријевић-Бранковић, Сузана; Којић, Весна; Радовановић, Жељко М.; Утицај јона сребра, бакра и цинка на својства биокерамичких материјала на бази калцијум-хидроксиапатита и калцијум-фосфата; Утицај јона сребра, бакра и цинка на својства биокерамичких материјала на бази калцијум-хидроксиапатита и калцијум-фосфата;


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