Genetic heterogeneity of primary open-angle glaucoma, and concentration changes of various inflammation and immune response mediators in the blood, aqueous humor or tissues, indicate the involvement of different genetic mechanisms and immune system activity in the pathogenesis of POAG. The aim of study was to determine the interdependent clinical, genetic and biochemical findings in POAG-HTG patients. Distribution of genetic polymorphisms for TNF-α -308 G/A and - 863 C/A in DNA samples was examined using PCR-RFLP, the concentrations of circulating TNF-α and HSP 70 in a plasma and concentration of sFas and sFasL in the aqueous humor were measured by commercial ELISA tests. Their association with quantitative clinical parameters was examined. This study included 357 subjects: 81 POAG-HTG, 35 PEXG, 77 with senile cataract and 164 healthy subjects age-sex matched. Furthermore, 35 samples of aqueous humor of POAG-HTG patients , 24 PEXG and 29 with senile cataract were treated. The results showed