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The rehabilitation of the experimental brain injury with hyperbaric oxygenation

dc.contributor.advisorJovanović, Tomislav
dc.contributor.otherStojiljković, Mirjana
dc.contributor.otherŠćepanović, Ljiljana
dc.contributor.otherRakić, Miodrag
dc.contributor.otherPeković, Sanja
dc.creatorBrkić, Predrag D.
dc.date.accessioned2016-01-05T12:05:57Z
dc.date.available2016-01-05T12:05:57Z
dc.date.issued2012-07-12
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=884
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:7289/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=44826895
dc.identifier.urihttp://nardus.mpn.gov.rs/123456789/2394
dc.descriptionUvod: Traumatske povrede centralnog nervnog sistema (CNS) predstavljaju širom sveta veliki zdravstveni problem, jer spadaju među vodeće uzročnike smrtnosti i različitih oblika invaliditeta u radno sposobnoj populaciji. Činjenica je da se samo u koordinisanim istraživanjima kako eksperimentalnim i tako kliničnim, mogu razviti novi kolaborativni modeli i nove strategije za sanaciju posledica moždane traume. U ovom kontekstu hiperbarična oksigenacija (HBO) se izdvaja kako procedura koja može u sinergističkom dejstvu sa drugim terapijama dovesti do ubrzanja oporavka posle moždane traume. Cilj ove studije je da utvrdi da li primena HBO može poboljšati oporavak pacova posle sukcione ablacije desnog senzomotornog korteksa. Materijal i metode: Deset nedelja stari mužijaci pacova Wistar soja raspoređeni su u sledeće grupe (n=8, po grupi): Kontrolna (K) intakne životinje, Kontrolna + HBO (KHBO) intakne životinje koje su prošle kroz HBO protokol, Šam (Š) lažno operisane životinje, Šam + HBO (ŠHBO), Lezija (L) desni sezomotorni korteks je uklonjen sukcionom ablacijom i L + HBO (LHBO). HBO protokol: pritisak 2,5 apsolutne atmosfere, 60 minuta, jedanput dnevno, tokom 10 dana. Za evaluaciju procesa oporavka lokomotornih funkcija korišćeni su test prelaska preko grede i merenje snage stiska. Količina malondialdehida (MDA), aktivnost superoksid dismutaze (SOD) i glutation peroksidaze (GPX) su mereni u uzorcima tkiva povređenog hemikorteksa. Postraumatski procesi su praćeni na uzorcima moždanog tkiva imunohistohemijskom metodom sa sledećim antitelima: GFAP, ED1, SMI31, GAP43 i SYP. U cilju evaluacije efekata HBO na proces neuronalne degeneracije sprovedeno je dovostruko imunofluorescentno bojenje: neuroni u procesu degeneracije su bojeni fluoro žadom B, dok je NeuN korišćen kao marker tela neurona...sr
dc.descriptionObjectives: Traumatic brain injuries (TBI) are one of the leading causes of death and chronic disability especially among the working population, and represent an important public health problem worldwide. It is proposed that coordinated research efforts of basic and clinical scientists will provide the opportunity to develop collaborative models for translational neuroscience and rehabilitation research. In this view hyperbaric oxygen therapy (HBO) appeared as an adjunctive therapy that has the synergistic effect with other treatments, strongly suggesting that combining therapies with HBO could yield better results than either alone. The aim of this study was to investigate whether HBO can improve the neurological recovery of the rat after suction ablation of the sensorimotor cortex. Methods: The experiments were conducted on the male Wister rats, 10 weeks old. Animals were organized into following groups (n = 8, per group): Control (C) intact animals, Control+HBO (CHBO) intact animals subjected to HBO treatment, Sham control (S) animals that underwent surgical procedure without damaging the brain tissue, Sham control+HBO (SHBO), Lesion group (L)–the right sensorimotor cortex was removed by suction ablation, Lesion+HBO (LHBO). HBO protocol: pressure applied 2.5 absolute atmospheres (ATA), for 60 minutes, once daily for 10 days. Beam walking test and grip strength meter were used to evaluate the recovery of motor functions. Malondialdehyde (MDA) content, activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX) sampled from injured hemicortex were measured. The posttraumatic processes were evaluated on the brain tissue preparations using the immunohistochemical method with antibodies: GFAP, ED1, SMI31, GAP43 and SYP...en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Медицински факултетsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41002/RS//
dc.rightsAutorstvo 3.0 Srbija (CC BY 3.0)
dc.sourceУниверзитет у Београдуsr
dc.subjecthiperbarična oksigenacijasr
dc.subjecthyperbaric oxygenationen
dc.subjectpovrede mozgasr
dc.subjectporemećaj lokomocijesr
dc.subjectoksidativni stressr
dc.subjectregeneracijasr
dc.subjectGAP43sr
dc.subjectsinaptofizinsr
dc.subjectastrogliozasr
dc.subjectneuronalna degeneracijasr
dc.subjectbrain injuryen
dc.subjectlocomotor impairmentsen
dc.subjectoxidative stressen
dc.subjectregenerationen
dc.subjectGAP43en
dc.subjectsynaptophysinen
dc.subjectastrogliosisen
dc.subjectneural degenerationen
dc.titleSaniranje eksperimentalne traumatske povrede mozga primenom hiperbarične oksigenacijesr
dc.titleThe rehabilitation of the experimental brain injury with hyperbaric oxygenationen
dc.typePhD thesis
dcterms.abstractЈовановић, Томислав; Стојиљковић, Мирјана; Ракић, Миодраг; Шћепановић, Љиљана; Пековић, Сања; Бркић, Предраг Д.; Санирање експерименталне трауматске повреде мозга применом хипербаричне оксигенације; Санирање експерименталне трауматске повреде мозга применом хипербаричне оксигенације;


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