Razvoj i validacija nove analitičke metode za pripremu uzoraka vode za kvantifikaciju policikličnih aromatičnih ugljovodonika primenom gasne hromatografije - masene spektrometrije
Докторанд
Ćirić, SlobodanМентор
Stankov-Jovanović, VesnaЧланови комисије
Mitić, VioletaStojanović, Gordana
Bojić, Aleksandar
Lolić, Aleksandar
Метаподаци
Приказ свих података о дисертацијиСажетак
Polycyclic aromatic hydrocarbons (PAHs) are organic compounds
whose structure consists of two or more condensed aromatic rings. Due
to their toxicity and persistence in the environment, PAHs monitoring
is very important especially in water, from where they can through the
food chain or directly reach humans. In this doctoral dissertation, a
method for the determination of PAHs in water, using gas
chromatography - mass spectrometry method was optimized and
validated. In the sample preparation technique, dispersive micro-solid
phase extraction, new sorbents, prepared by thermal and chemicalthermal
modification of natural zeolite, clinoptilolite, were used. After
optimization and validation experiments, it was concluded that
applying 200 mg of acid-thermal modification (prepared modifying
clinoptilolite using 1.5 mol L-1 hydrochloric acid and thermally treated
at temperature of 500 oC) enabled achieving the best analytical
characteristics. Efficiency values ranged from 69%
(dibenzo[a,h]an...thracene) to 123% (pyrene). The obtained analytical
parameters of the method validation were within the validation
criterias. The optimized and validated method was used for analysis of
real water samples of the river Nišava and its tributaries in the territory
of municipalities Dimitrovgrad, Pirot, Bela Palanka and Niš.
Naphthalene, acenaphthylene, acenaphthene and fluorene were present in all samples, and the total concentration of PAHs varied depending
on the sampling site, and was in the interval of 6.47 - 33.6 μg L-1, where
tributaries contributed to the increase of the total PAHs concentration
in Nišava. The obtained individual and total concentrations were higher
than the limits prescribed by official regulations and it was concluded
that the river Nišava in the territory of Serbia has been contaminated
with PAHs, of which light PAHs were predominantly presented in all
samples.