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Protective effects of probiotics in the model of cadmium toxicity in rats

dc.contributor.advisorĐurašević, Siniša
dc.contributor.otherPavlović, Slađan Z.
dc.contributor.otherJasnić, Nebojša
dc.creatorJama, Adel Masoud
dc.date.accessioned2016-01-05T11:48:18Z
dc.date.available2016-01-05T11:48:18Z
dc.date.available2020-07-03T08:11:02Z
dc.date.issued2013-11-20
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/2149
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=1120
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:7877/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024615090
dc.description.abstractKadmijum je široko prisutni toksikant životne sredine, koji izaziva različite poremećaje u biološkim sistemima, uklјučujući tu i bubrežnu disfunkciju i degeneraciju tkiva jetre. Sa druge strane, pretpostavlјa se da su pozitivne osobine probiotskih bakterija u vezi sa njihovom sposobnošću da vezuju različite ligande, što dovodi do pobolјšanja crevne mikrobijalne ravnoteže i drugih koristi za domaćina. Imajući u vidu pomenuta, ova studija je sprovedena da istraži zaštitni efekat probiotika protiv kadmijum-indukovanog toksičnosti kod pacova. Muški pacovi Rattus norvegicus Wistar soja, težine 130±10 g, gajeni su u standardnim laboratorijskim uslovima (22±2°C, režim 12h svetlo/12h mrak) u metaboličkim kavezima (3 pacova/kavezu). Životinje su podeljene u četiri grupe: 1) kontrolne jedinke; 2) životinje tretirane probioticima; 3) životinje koje su primale kadmijum u formi CdCl2; i 4) životinje tretirane probioticima i kadmijumom. U krvi je određivana koncentracija kadmijuma, aktivnost alanin aminotransferaze (ALT) i aspartat aminotransferaze (AST), kao i nivoi adrenalina i noradrenalina. U homogenatima tkiva jetre i bubrega određivane su koncentracije kadmijuma, cinka i bakra, kao i aktivnosti ukupne superoksid dismutaze (SOD) i katalaze (CAT). Takođe, ispitivan je i histomorfološki profil tkiva jetre i bubrega u svim grupama životinja. U fecesu eksperimentalnih životinja određivana je koncentracija kadmijuma i mikrobijalni profil odabranih vrsta bakterija crevne mikroflore. Na kraju, Comet testom je testirana genotoksičnost kadmijuma in vitro and in vivo. Na osnovu naših rezultata mogu se izvesti sledeći zaključci:Davanje kadmijuma eksperimentalnim životinjama u formi kadmijum hlorida i koncentraciji 70ppm u pijaćoj vodi izaziva značajno smanjenje u porastu telesne mase tokom 5 nedelja eksperimenta...sr
dc.description.abstractCadmium is a ubiquitous environmental toxicant that causes a variety of disturbances in biological systems, including renal dysfunction and liver tissue degeneration. On the other hand, it is supposed that beneficial properties of probiotic bacteria are related to their capacity to adhere or bind different targets, thus leading to improved intestinal microbial balance and other benefits to host. Bearing aforementioned in mind, the present study was undertaken to investigate the protective effect of probiotic supplementation against cadmium-induced toxicity in rat. Male Wistar rats Rattus norvegicus, weighing 130±10 g, were acclimated to 22±2°C in metabolic cages (3 rats/cage) and maintained under a 12h light/12h dark cycle. Animals were divided into four groups: 1) controls; 2) probiotics treated; 3) CdCl2 treated; and 4) probiotics + CdCl2 treated. The cadmium concentrations were obtained in the blood, liver, kidney, and feces, as well as the blood alanine aminotransferase (ALT), aspartate aminotransferase (AST), adrenaline and noradrenaline activities. Furthermore, histomorphological changes of liver and kidney were determined, as well as the level of total supeoxide dismutase (SOD) and catalase (CAT) activities, and zinc and cooper concentration. Finally, cadmium genotoxicity were determined both in vitro and in vivo by Comet test, as well as the alterations in the fecal microflora content. Based on our results we can conclude that:1. Exposure of rats to cadmium chloride (70ppm) alone resulted in significant decrease in body weight gain compared with the control group...en
dc.formatapplication/pdf
dc.languageen
dc.publisherУниверзитет у Београду, Биолошки факултетsr
dc.rightsopenAccessen
dc.sourceУниверзитет у Београдуsr
dc.subjectpacovsr
dc.subjectprobioticsen
dc.subjectprobioticisr
dc.subjectkadmijumsr
dc.subjectlaktobacilisr
dc.subjectbifidobakterijesr
dc.subjectalanin aminotransferazasr
dc.subjectaspartat aminotransferazasr
dc.subjectsuperoksid dismutazasr
dc.subjectkatalazasr
dc.subjectadrenalinsr
dc.subjectnoradrenalinsr
dc.subjectcadmiumen
dc.subjectLactobacillusen
dc.subjectBifidobacteriumen
dc.subjectalanine aminotransferaseen
dc.subjectaspartate aminotransferaseen
dc.subjectsuperokside dismutaseen
dc.subjectcatalaseen
dc.subjectadrenalineen
dc.subjectnoradrenalineen
dc.titleZaštitni efekat probiotika na modelu toksičnosti kadmiuma kod pacovasr
dc.titleProtective effects of probiotics in the model of cadmium toxicity in ratsen
dc.typedoctoralThesis
dc.rights.licenseBY-NC-ND
dcterms.abstractЂурашевић, Синиша; Павловић, Слађан З.; Јаснић, Небојша; Јама, Aдел Масоуд; Заштитни ефекат пробиотика на моделу токсичности кадмиума код пацова; Заштитни ефекат пробиотика на моделу токсичности кадмиума код пацова;
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/2434/Disertacija.pdf


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