Biohemijska i fiziološka karakterizacija klonovatopole (Populus spp.) u procesu fitoekstrakcije bakra, nikla i kadmijuma

Abstract

Predmet ovog istaživanja bio je ispitivanje uticaja jona tri teška metala (Ni2+, Cu2+ i Cd2+) u dve toksične koncentracije u zemljištu na fiziološke i biohemijske karakteristike odabranih klonova topola, M1, B229 i Pe 19/66. Ispitan je i potencijal odabranih klonova topola da vrše fitoekstrakciju-akumulaciju teških metala iz zemljišta u svoje nadzemne delove što dovodi do dugoročnog uklanjanja ovih perzistentnih polutanata iz životne sredine. Takođe, ispitan je uticaj teških metala na antioksidantni potencijal, sposobnost neutalizacije slobodnih radikala, aktivnosti antioksidantih enzima kao i na sadržaj slobodnih i konjugovanih poliamina (Put, Spm, Spd), određenih HPLC analizom, i sadržaj biljnih hormona poput indol-3-sirćetne kiseline i abscisinske kiseline, određenih GC/MS analizom, u listovima i korenovima klonova topola.

The aim of this study was to estimate and compare phytoextraction capacities of three poplar clones (M1, B229 and Pe 19/66) in soil. Furthermore, the goal was to assess different biological responses among the poplar clones during exposure to different concentration of three heavy metal ions (Ni2+, Cu2+ i Cd2+). In order to track changes in poplars’mineral, physiological, biochemical and antioxidant status during the abiotic stress, quantification of physiological properties, free and conjugated polyamines, total phenolics as well as quantification of phytohormones (indol-3-acetic and abscisic acid) was done. Furthermore, assessment of antioxidant potential by tracking radical scavenger capacities (RSC) against DPPH, ABTS, OH and NO radicals and by measuring enzymes activities (SOD, GSH-Px, GPx, GR) in vitro was performed in root and leaves of poplar clones.