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Значај структурних и функционалних промена хороидног плексуса миша у иницијацији неуроинфламације

dc.contributor.advisorVandenbroucke, Roosmarijn
dc.contributor.otherKanazir, Selma
dc.contributor.otherNedeljković, Nadežda
dc.contributor.otherAnđus, Pavle
dc.contributor.otherLibert, Claude
dc.creatorBrkić, Marjana
dc.date.accessioned2020-02-14T14:30:25Z
dc.date.available2020-02-14T14:30:25Z
dc.date.available2020-07-03T08:08:07Z
dc.date.issued2019-09-27
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/11836
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=7215
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:20932/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51822095
dc.description.abstractNeuroinflammation has been considered a common denominator and crucial player in neurodegeneration observed in various central nervous system (CNS) disorders. Considering the increasing evidence on the role of choroid plexus (CP) in neuroinflammatory processes through alterations in morphology and functionality of the choroid plexus epithelial (CPE) cells, the main goal of this thesis was to test the contribution of the CP in the initiation of neuroinflammation in two animal models of neuroinflammation-associated diseases: a model of Alzheimer’s disease, induced by intracerebroventricularly (i.c.v.) injected amyloid beta oligomers (AβO) and a lipopolysaccharide (LPS) induced sepsis animal model of systemic inflammation. Main findings from this research conclude that blood cerebrospinal fluid barrier (BCSFB) permeability is increased upon AβO injection, resulting from the loss of typical cuboidal morphology of CPE cells and a decrease in expression of tight junctions components. In the CP, upregulation of gene expression for various cytokines was observed, as well as their increased levels in the cerebrospinal fluid (CSF). Also, increase in gene expression for several matrix metalloproteinases (MMP) in the CP, and in MMP activity in the CSF was noted. After i.c.v. injection of broad spectrum MMP inhibitor with AβO, prevention of AβO-induced BCSFB permeability was found. In accordance with this, increase in BCSFB permeability upon AβO injection was observed in MMP3 deficient mice, but to a lesser extent. Furthermore, an increase in the number of particles in the CSF and an increase in gene expression of extracellular vesicles (EV) markers and miR-155 was found in the CP. A similar pattern of changes in the CP was observed in response to LPS injection compared to AβO injection. The results of this study revealed a significant role for the CP in the initiation of neuroinflammation, through structural and functional changes, in two different models associated with neuroinflammation.sr
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Биолошки факултетsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173056/RS//
dc.rightsopenAccessen
dc.sourceУниверзитет у Београдуsr
dc.subjectBCSFBsr
dc.subjectKLBen
dc.subjectCPsr
dc.subjectMMPsr
dc.subjectEVsr
dc.subjectAβOsr
dc.subjectneuroinflammationsr
dc.subjectsepsissr
dc.subjectHPen
dc.subjectMMPen
dc.subjectEVen
dc.subjectAβOen
dc.subjectneuroinflamacijaen
dc.subjectsepsaen
dc.titleStudies on structural and functional changes of mouse choroid plexus in the initiation of neuroinflammationsr
dc.title.alternativeЗначај структурних и функционалних промена хороидног плексуса миша у иницијацији неуроинфламацијеen
dc.typedoctoralThesis
dc.rights.licenseBY-NC-SA
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/1806/Disertacija.pdf
dc.identifier.fulltexthttp://nardus.mpn.gov.rs/bitstream/id/1807/IzvestajKomisije21878.pdf


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