Ekspresija mamaglobina u tkivu kao prediktora agresivnosti tumora dojke

Abstract

Uvod: Humani mamaglobin je jedan od naznačajnijih markera hematogene diseminacije karcinoma dojke. Naš cilj je ukazati na važnu ulogu peritumorskog tkiva kao aktivnog učesnika u procesu tumorogeneze i koncentraciju/ekspresiju mamaglobina u peritumorskom tkivu kao značajnog prognostičkog faktora. Materijal i metode: Ova studija je obuhvatila 64 pacijentkinje sa primarnim karcinomom dojke tokom perioda od pet godina. Za određivanje koncentracije mamaglobina A u tkivu karcinoma i peritumorskom tkivu koristili smo ELISA test, dok je za određivanje relativne genske ekspresije ovog molekula korišćen qRT-PCR. Rezultati: Koncentracija mamaglobina A raste kako u tkivu karcinoma tako i u peritumorskom tkivu sa porastom veličine tumora, broja zahvaćenih limfnih čvorova, broja metastaza, dok je relativna ekspresija Mamaglobina A statistički značajno viša u karcinomu nego u peritumorskom tkivu, bez obzira na veličinu tumora, broj zahvaćenih limfnih čvorova, broj metastaza i tumorski tip. Koncentracija mamaglobina A je veća u peritumorskom tkivu nego u tkivu duktalnog karcinoma, dok je u slučaju lobularnog karcinoma koncentracija mamaglobina A veća u karcinomu nego u peritumorskom tkivu. Zaključak: Koncentracije Mamaglobina A u peritumorskom tkivu veće od 0,6704221 ng/ml i u tkivu karcinoma veće od 0,5784426 ng/ml, kao i relativna genska ekspresija Mamaglobina A u tkivu karcinoma veća od 1.003 su cut-off vrednosti na osnovu kojih se mogu identifikovati pacijenti koji su pod povećanim rizikom za razvoj metastatske bolesti i koji se mogu tretirati ranim radikalnim adjuvantnim lečenjem.

Introduction: Human mammaglobin is considered to be one of the most significant markers of hematogenous dissemination of breast carcinoma. Our goal is to indicate the important role of peritumoral tissue as an active participant in the tumorrogenesis process and the concentration / expression of mamaglobins in the peritumoral tissue as a significant prognostic factor. Materials and methods: This research included 64 patients with primary breast carci-noma during five-year follow-up period. To determine the concentration of mamaglobin A in samples of carcinoma tissue and peritumoral tissue, ELISA essay was used, and for the determination of relative gene expression of Mammaglobin A, qRT-PCR was used. Results: The concentration of mamaglobin A increases in both the carcinoma tissue and peritumoral tissue with an increase in tumor size, number of affected lymph nodes, number of metastases, while relative expression of Mamaglobin A is statistically significantly higher in carcinoma tissue than in peritumoral tissue, regardless of tumor size, number of affected lymph nodes, number of metastases and tumor type. The concentration of mamaglobin A is higher in peritumor tissue than in tissue of ductal carcinoma, while in the case of lobular carcinoma the concentration of mamaglobin A is higher in carcinoma tissue than in peritumor tissue. Conclusion: Mammaglobin A concentration in peritumoral tissue higher than 0,6704221 ng/ml, and in carcinoma tissue higher than 0,5784426 ng/ml, as well as Mammaglobin A relative gene expression in carcinoma tissue higher than 1.003 were determined as cut-off values that may identify patients who are at higher risk of metastatic disease, which would be treated with early radical adjuvant treatment.