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Analysis of the indicators of the oxidative stress in adolescent girls with polycystic ovary syndrome on different oral hormonal contraception therapy regiments

dc.contributor.advisorMacut, Đuro
dc.contributor.otherSavić-Radojević, Ana
dc.contributor.otherVujović, Svetlana
dc.contributor.otherPlješa-Ercegovac, Marija
dc.contributor.otherBjelica, Artur
dc.creatorMažibrada, Ilijana I.
dc.date.accessioned2019-02-08T15:49:33Z
dc.date.available2019-02-08T15:49:33Z
dc.date.issued2018-09-19
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=6584
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:19368/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50764303
dc.identifier.urihttp://nardus.mpn.gov.rs/123456789/10736
dc.description.abstractSindrom policističnih jajnika (PCOS) je složeni poremećaj prisutan u 6-10% žena reproduktivnog perioda, a karakteriziran je hiperandrogenizmom, hiperinsulinemijom, oštećenom funkcijom hipotalamo-hipofizno-ovarijumske osovine i disfunkcijom hormonske aktivnosti masnog tkiva. Pretpostavlja se da različiti endokrini i metabolički poremećaji mogu dodatno uticati na pojavu oksidativnog stresa kod žena sa PCOS a posebno hiperandrogenizam, koji se smatra glavnim pokretačem povećane produkcije reaktivnih kiseoničnih vrsta (ROS). Kod adolescentkinja sa PCOS, oralna hormonska kontraceptivna (OHK) pilula može efikasno poboljšati biohemijske i kliničke parametre. Cilj ovog rada bio je određivanje parametara oksidativnog stresa i aktivnost ključnih enzima antioksidantne zaštite kod adolescentkinja sa PCOS, koje su bile normalno uhranjene i bez poremećaja tolerancije glukoze, pre i posle primene oralne hormonske kontraceptivne pilule. Pored toga, ispitali smo markere inflamacije, hs-CRP i fibrinogen kao i njihovu vezu sa antropometrijskim i parametrima lipidnog statusa. Materijal i metode: Studijom je praćeno 35 adolescentkinja sa PCOS i 17 zdravih kontrola, odgovarajuće starosti i indeksa telesne mase. Izvršena su antropometrijska merenja, biohemijska i hormonska ispitivanja. Parametri oksidativnog stresa, koncentracije nitrotirozina i proteinskih tiol grupa kao i aktivnosti glutation-peroksidaze i superoksid dismutaze, određivane su našte i tokom oralnog testa opterećenja glukozom (OGTT). Genetski polimorfizmi glavnih citosolnih glutation S-transferaza (GST) određeni su PCR-om. Insulinska rezistencija procenjivana je primenom homeostatskog indeksa rezistencije na insulin (HOMA-IR), homeostatskog indeksa funkcije β ćelija (HOMA-β), insulinogenog indeksa (IGI), “Matsuda” indeksa sezitivnosti na insulin [ISI(Matsuda)] i AUC glukoze. Rezultati: U uslovima našte, nije pronađena značajna razlika u koncentraciji parametara oksidativnog stresa između adolescentkinja sa PCOS i zdravih kontrola. Akutna hiperglikemija tokom OGTT izazvala je značajne promene u koncentraciji parametara oksidativnog oštećenja proteina kod ispitanica sa PCOS. Posebno su promene koncentracije nitrotirozina bile povezane sa testosteronom, DHEAS, androstenedionom, FAI i LH, dok je promena proteinskih tiol grupa bila povezana sa DHEAS (p<0,05)...sr
dc.description.abstractPolycystic ovary syndrome (PCOS) is a complex disorder present in 6-10% of the reproductive age women and characterized by hyperandrogenism, hyperinsulinemia, impaired function of the hypothalamic-pituitary-ovarian axis and dysfunction of adipose tissue hormonal activity. It has been supposed that different endocrine and metabolic disorders can further affect the occurrence of oxidative stress in women with PCOS, with special emphasis on hyperandrogenism which is considered the main progenitor of increased production of reactive oxygen species (ROS). In an adolescent with PCOS, oral contraceptive therapy can effectively improve biochemical and clinical parameters. The aim of this study was to determine oxidative stress by-products and activity of key antioxidant enzymes in adolescent girls with PCOS, which were non-obese and normoinsulinemic, before and after oral contraceptive therapy. In addition, we evaluated inflammatory markers, high-sensitivity C-reactive protein (hs-CRP) and fibrinogen as well as their relationship with anthropometric and lipid parameters. Methods: The study comprised a total of 35 adolescent girls with PCOS and 17 healthy controls, appropriate age and body mass index. The anthropometric measurements, biochemical and hormonal testing were performed. Oxidative stress parameters, nitrotyrosine and thiol groups’ concentrations as well as glutathione peroxidase and superoxide dismutase activities were determined under fasting conditions and during oral glucose tolerance test (OGTT). Glutathione S-transferases (GSTs) polymorphisms were determined by PCR. Insulin resistance was assessed by the homeostasis model assesment of insulin resistance (HOMA-IR), homeostasis model assessment of β-cell function (HOMA-β), insulinogenic index (IGI), insulin sensitivity index-Matsuda [ISI(Matsuda)] and blood glucose area under the curve (AUCglucose). Results: Under fasting conditions, no significant difference of oxidative stress parameters was found between adolescent patients with PCOS and healthy controls. However, acute hyperglycemia during OGTT induced significant alteration in parameters of oxidative protein damage in PCOS patients...en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Медицински факултетsr
dc.rightsAutorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
dc.sourceУниверзитет у Београдуsr
dc.subjectSindrom policističnih jajnika; oksidativni stres; C-reaktivni protein; fibrinogen; inflamacijasr
dc.subjectPolycystic ovary syndrome; Oxidative stress, C-reactive protein; Fibrinogen; Inflammationen
dc.titleAnaliza pokazatelja oksidativnog stresa u adolescentkinja sa sindromom policističnih jajnika na različitim terapijskim režimima oralne hormonske kontracepcijesr
dc.title.alternativeAnalysis of the indicators of the oxidative stress in adolescent girls with polycystic ovary syndrome on different oral hormonal contraception therapy regimentsen
dc.typePhD thesis


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